Box-Benhnken试验设计优化五味子木脂素提取工艺

Optimization of extraction of lignans by Box-Benhnken design from Schisandra Chinensis

目的利用Box-Benhnken响应曲面试验设计对五味子中木脂素成分进行超声提取工艺研究。方法根据Box-Benhnken设计原理,以五味子中总木脂素得率为响应值,分析超声提取过程中各因素及其交互作用,以及对五味子总木脂素得率的影响。结果五味子中木脂素最佳超声提取条件为以纯甲醇为溶媒,液料比55 m L/g,超声提取时间35 min。在此试验条件下,五味子中木脂素得率可达5.49%。结论本实验所建立的数学模型极显著(P<0.01),可用于对五味子中木脂素超声提取工艺研究。

Objective To optimize the extraction technique of lignans by Box-Benhnken design from Schisandra chinensis. Methods Base on Box-Benhnken design,the content of total lignans in Schisandra chinensis was used as response value and the factors and their interactions in the course of ultrasonic extraction were analyzed. Results The optimized ultrasonic method of lignans was as follows： pure methanol,liquid-solid ratio of 55 m L / g,ultrasonic extraction time of 35 min. Under these conditions,the yield of total lignans was up to 5. 49%. Conclusion The mathematical model that the present study established was very significant（ P〈0. 01）,and it might be applied to analysis and prediction the extraction technique of lignans in Schisandra chinensis.