何首乌饮对衰老大鼠肾脏CD26表达的影响

目的:探讨CD26在自然衰老大鼠肾组织中的表达,及何首乌饮对自然衰老大鼠肾组织CD26的影响。方法 :Wistar大鼠动分组:青年对照组(12月龄)大鼠10只,自然衰老对照组(18月龄)大鼠10只,何首乌饮干预组(18月龄)大鼠10只,何首乌饮灌胃60天。免疫组化法观察CD26在肾脏分布。结果:何首乌饮干预组的肾小球数目明显高于自然衰老对照组(P<0.01),与青年组接近;CD26在老年对照组大鼠肾组织的表达较青年对照组明显增强,何首乌饮干预组其表达显著下降(P<0.05)。结论:何首乌饮减弱自然衰老大鼠肾脏CD26的表达,具有延缓衰老作用。

Objective：To investigate the expression of CD26 in the kidney tissues of aging rats and the effects of Heshouwuyin on tire renal CD26 expression. Methods：Wistar rats were divided into three grnups：natural young group （NY group, 12 months, n=10）,natnral aging model group（NA group, 18mongths, n=10）and Hestlouwuyin-treated aging group （HT group, 18months. n=10）. NA wistar rats were fed with 0.9% NaCl.HT wistar rats were fed with Heshouwuyin for sixty days. The slices of kidney were made by histological method and stained by HE staining.The morphological changes of kidney were observed under microscope and quantitatively analyzed.The expression of CD26 in kidney was examined by using immunohistochemistry. Results The kidney glomerulus number of rats in HT gronp was higher than in N A group and approach NY rats （P 〈 0.01）;the renal capsule breadth and the kidney glomerulus diameter were obviously lower than NA group （P〈0.01）.Compared with NY group, CD26 were significantly increased in NA group（P〈0.01）.CD26 in renal tissue was mainly expressed in T lymphncyles of renal interstitium.CD26 expression in NA group was significantly higher than that in NY group,and also higher Ihan that in HT group （P〈0.01）.In lit group,CD26＋T lymphocytes infiltration of renal interstitium was positively correlate NY group. Conclusions：CD26 is related with aging nephropathy.Heshouwuyin maybe inhibit T lymphocytes infiltration to reduce the expression of CD26 in renal interstitium,thus protecting the kidney function and delay renal senility of rats.