宁夏回、汉族脂联素基因+45位点多态性及其血清水平与代谢综合征的相关性

Relationships between adiponectin gene + 45 nucleotide T / G polymorphism and serum adiponectin level with MS in Hui and Han nationalities in Ningxia

目的探讨宁夏回、汉族人群脂联素(APN)基因+45位核苷酸T/G多态性与代谢综合征(MS)的相关性。方法收集回族MS患者207例(MS组)、正常对照组98例,和汉族MS患者136例(MS组)、正常对照组102例(对照组),采用聚合酶链反应-限制性片段长度(PCR-RFLP)多态性技术和酶联免疫吸附法(ELISA),对各组APN基因+45位核苷酸T/G多态性及其血清APN浓度进行检测。结果回、汉族MS组间与对照组间基因型频率及等位基因频率均差异有统计学意义(P<0.05)。回族MS组GG基因型频率及G等位基因频率高于回族对照组(均P<0.05)。汉族MS组与汉族对照组间基因型频率及等位基因频率差异均无统计学意义(P>0.05)。回、汉族MS组血清APN水平均低于其对照组(P<0.05),回族TG+GG基因型血清APN水平低于TT基因型者,汉族TT基因型血清APN水平低于TG+GG基因型者。Logistic回归分析显示,APN基因45T/G多态性是回族MS的危险因素。结论 APN基因+45T/G多态性在回、汉族人群之间存在差异;回、汉族MS组血清APN均低于正常人群;APN基因+45T/G多态性与宁夏回族人群MS发病具有相关性,与汉族MS无相关性。

Objective To investigate relationships between adiponectin（ APN） gene ＋ 45 nucleotide T/G polymorphism and serum APN level with metabolic syndrome（ MS） in Hui and Han nationalities in Ningxia. Methods 136 Han patients with MS as MS group,as well as Hui healthy people（ n = 98） and Han healthy people（ 102） as control group were included the study. Polymerase chain reaction- restriction fragment length polymorphism（ PCR- RFLP） and enzyme- linked immunosorbent assay（ ELISA） were used to detect the gene polymorphism of APN and the serum APN level. Results The frequencies of genotype and allele were significantly different between Hui MS group and Han MS group（ both P 〈0. 05）,and there were also significant differences between Hui control group and Han control group. The frequencies of GG genotype and G allele in Hui MS group were higher than those in Hui control group（ both P 〈0. 05）. The frequencies of genotype and allele were not significantly different between Han MS group and Han control group（ both P 〈0. 05）. Compared with the control group,the serum APN level in MS group was lower in both Hui and Han nationalities（ both P 〈0. 05）. The serum APN level in subject with TG ＋ GG genotypes was lower than that with TT genotype in Hui nationality（ P 〈0. 05）.The serum APN level in subject with TT genotypes was lower than that with TG ＋ GG genotype in Han nationality（ P 〈0. 05）. Logistic regression analysis showed that APN ＋ 45 T / G gene polymorphisms were the risk factors for metabolic syndrome in Hui subjects.Conclusion There is racial difference between Hui and Han nationalities with APN ＋ 45 gene polymorphism. The serum APN level in MS patients is reduced in both Hui and Han nationalities,and APN ＋ 45 T / G polymorphism may be associated with MS in Hui nationalities but not in Han nationalities in Ningxia.