红花注射液疑似致敏蛋白的初步研究

Preliminary Study on Suspected Hypersensitive Protein of Carthamus tinctorius Injection

目的:研究红花注射液致敏反应相关蛋白。方法:收集有/无致敏性红花注射液各3批,经离子交换树脂梯度洗脱富集其中的总蛋白,利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法分离蛋白并初步分析其差异。结果:40 k D和26 k D处,有致敏性红花注射液各有1个颜色较深、轮廓清晰的蛋白条带;10~17 k D处,无致敏性红花注射液有1个颜色较深、轮廓清晰的蛋白条带。结论:40 k D和26 k D处的蛋白可能是红花注射液致敏的原因之一,而17 k D处的蛋白可能不会引起过敏反应。致敏蛋白的分子结构及含量限度等仍需进一步研究。

OBJECTIVE： To study the hypersensitive reaction-related protein of Carthamus tinctorius injection. METHODS： Three batches of C. tinctorius injection with or without hypersensitive reaction were collected, and the proteins of C. tinctorius in- jection were separated and enriched by gradient elution of ion exchange resin; sodium dodecyl sulfate-polyacrylamide gelelectropho- resis （SDS-PAGE） was used to separate the proteins and preliminarily analyze the differences of them. RESULTS： The allergenic C. tinctorius injection had two deep-color, clear-cut bands, of which the protein molecular weight were 40 kD and 26 kD, respec- tively. Non-allergenic C. tinctorius injection have only one deep-color, clear-cut band, of which the protein molecular weight was 10-17 kD. CONCLUSIONS： The 40 kD and 26 kD protein are probably one of the reasons of C. tinetorius injection sensitization, but the 17 kD protein may not cause allergic reactions. The molecular struc^re and content limit of sensitization protein still needs further analysis.