酸枣仁汤对睡眠剥夺模型大鼠学习记忆的影响

Effects of Ziziphus jujuba Decoction on the Learning and Memory in Sleep Deprivation Model Rats

目的:研究酸枣仁汤对睡眠剥夺模型大鼠学习记忆的影响。方法:采用改良多平台法复制大鼠睡眠剥夺模型。50只SD大鼠随机均分为正常对照(等容蒸馏水)组、模型(等容蒸馏水)组与酸枣仁汤高、中、低剂量[21.00、10.50、5.25 g(生药)/kg]组,复制模型的同时ig给药,每天1次,连续7 d。观察大鼠一般状态,测定体质量;Y型迷宫测定大鼠错误反应次数与主动回避率;检测大鼠海马组织乙酰胆碱酯酶(ACh E)、乙酰胆碱转移酶(Ch AT)活性与血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。结果:与正常对照组比较,模型组大鼠一般状态欠佳,体质量减少;错误反应次数增加,主动回避率降低;海马组织ACh E活性增强、Ch AT活性减弱,血清SOD活性减弱、MDA含量增加,差异均有统计学意义(P<0.01或P<0.05)。与模型组比较,酸枣仁汤高剂量组大鼠体质量增加;错误反应次数减少,主动回避率增加;高、中剂量组大鼠海马组织ACh E活性减弱、Ch AT活性增强,血清SOD活性增强;高、中、低剂量组大鼠血清MDA含量减少,差异均有统计学意义(P<0.01或P<0.05)。结论:酸枣仁汤能改善睡眠剥夺模型大鼠的学习记忆下降,其机制可能与保护胆碱能系统和抗氧化作用有关。

OBJECTIVE： To study the effects of Ziziphus jujuba decoction on the learning and memory in sleep deprived rats. METHODS： The model rats of sleep deprivation were established with modified multiple platform method. 50 SD rats were random- ly divided into normal control （isovolumic distilled water） group, model （isovolumic distilled water） group and Z. jujuba decoc- tion high-dose, medium-dose and low-dose [21.00, 10.50, 5.25 g （medicinal materials）/kg] groups. Replication model was conduct-ed with intragastric administration for 7 d, once a day. General conditions of rats were observed, and body mass was weighed. The error response times and active avoidance rate of rats were tested byY-maze. The activity of ChAT and AChE in rat hippocampal, SOD activity and MDA content in rat serum were determined. RESULTS： Compared with normal control group, rats had poor gen- eral conditions, the weight decreased, the error response times increased, the active avoidance rate decreased, AChE activity in- creased, ChAT activity in hippocampal and SOD activity in serum decreased, and MDA content in serum increased in model group. There was statistical significant difference （P〈0.01 or P〈0.05）. Compared with model group, weight of rats increased, active avoidance rate and the error response times decreased in Z jujuba decoction high-dose group. AChE activity in hippocampal de- creased, ChAT activity in hippocampal and SOD activity in serum increased in Z. jujuba decoction high-dose group and medium- dose group, the MDA content in serum decreased in Z. jujuba decoction high-dose group, medium-dose and low-dose groups. There was statistical significant difference （P〈0.01 or P〈0.05）. CONCLUSIONS： Z jujuba decoction can improve the learning and memory in model rats after sleep deprivation. It is possibly related to the protection of cholinergic system and its antioxidant effect.