摘要
研究了5-羟色胺(5-HT)对马氏珠母贝解剖获得卵母细胞的促熟作用及5-HT受体(5-HTpf)在其性腺中的分布情况。通过体外浸泡的方法处理马氏珠母贝卵母细胞使其成熟,结果表明一定浓度的5-HT可显著促使马氏珠母贝卵母细胞生发泡破裂,并使已经发生生发泡破裂的卵母细胞进一步成熟,同时可诱导5-HT受体基因的表达。用原位杂交的方法对5-HT受体在马氏珠母贝性腺中的分布的研究结果揭示,卵巢中次级卵母细胞和卵细胞均存在5-HT受体,有阳性信号出现;精巢中仅滤泡壁及精母细胞中有少量表达。以上结果表明马氏珠母贝的卵母细胞不仅能产生5-HT并且可表达5-HT受体。
To investigate the effect of 5-HT on the maturation of oocyte stripped from the pearl oyster Pinctada fucata,oocytes were treated with 5-HT solution in vitro. Results showed that 5-HT was able to induce not only the Germinal Vesicle Breakdown（GVBD） of the oocytes remarkably, but also the maturation of the oocytes already GVBD. Gene expression of the 5-HT receptor was able to be induced at the same time. Distribution of 5-HT receptor（5-HTpf） within gonads of P.fucata was also studied using in situ hybridization（ISH） technique. The results revealed that 5-HT receptor 5-HTpfexisted in the oocytes of different development stages and in the spermatocytes, and the reactive substances of 5-HTpfdistributed along the cytoplasm and membrane of oocytes and showed blue. The above results indicate that the oocytes of P.fucata can not only produce 5-HT, but also express its receptor.
出处
《海洋通报》
CAS
CSCD
北大核心
2015年第1期89-94,101,共7页
Marine Science Bulletin
基金
国家863课题(2012AA10A410)
广东省海洋渔业科技推广专项(A201301A03)
