摘要
目的:探讨中药止痛消结汤治疗大鼠乳腺增生病的作用机理。方法:将60只SD大鼠随机将分成6组;肌注苯甲酸雌二醇合黄体酮注射液建立大鼠乳腺增生模型;分组ig给药30d;测量各组大鼠乳头高度;免疫组化法测定血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(b FGF)的表达水平;镜下观察病理组织学变化。结果:模型组乳头高度明显高于正常组(P<0.05),治疗后高中剂量组、乳癖消组均低于模型组(P<0.05),低剂量组与模型组比较变化不明显(P>0.05);与正常组比较,模型组VEGF、b FGF表达水平明显增高(P<0.05),高中剂量组、乳癖消组均低于模型组(P<0.05),低剂量组与模型组比较变化不明显(P>0.05);高中剂量组、乳癖消组与模型组比较增生改善显著(P<0.05或P<0.01)。结论:止痛消结汤高中剂量组对大鼠乳腺增生有明显的干预作用。
Objective:To discuss the mechanism of Zhitong Xiaojie Decoction in treating the rats with breast hyperplasia.Methods:60 SD rats were randomly divided into 6 groups.The MGH model was established by injecting rats with estradiol benzoatis and progesterone.The treatment groups were treated with Zhitong Xiaojie Decoction for 30 days.The height of teats,levels of VEGF and b FGF were measured with immunohistochemical method and the changes of histological structure of the mammary glands were examined.Results:The height of teats in the model control group were obviously higher than that in the normal group(P〈0.05).The height of teats in the high-level,the middle-level and Rupixiao control group were all lower than that in the model control group(P〈0.05).The height of teats in the low-level control group was similar to that in the model control group(P〈0.05).It showed that the levels of VEGF and b FGF in the model control group were significantly higher than that in the normal group(P〈0.05 or P〈0.01) and the levels of VEGF and b FGF in the high-level,the middle-level and Rupixiao control group were all lower than that in the model control group(P〈0.05).The levels of VEGF and b FGF in the low-level control group was similar to that in the model control group(P〉0.05).Compared to the model group,the level of mammary hyperplasia was evidently lower than that in the high-level,the middle-level and Rupixiao control group(P〈0.05 or P〈0.01).Conclusion:Zhitong Xiaojie Decoction has significant intervention in rats with experimental breast hyperplasia.
出处
《辽宁中医杂志》
CAS
北大核心
2015年第2期408-410,I0002,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
陕西省科学技术厅[陕科计发(2009)82号文件]
中药现代化项目[2009K19-03(6)]