贝母合剂对肺损伤大鼠血管内皮功能的干预作用

Intervention Effect of Fritillaria Mixture on Endothelial Function in Rats with BLM-induced Lung Injury

目的:探讨贝母合剂对博来霉素肺损伤大鼠血管内皮功能的干预作用。方法:40只大鼠随机分为4组:假手术组、模型组、地塞米松组和贝母合剂组,后3组建立博来霉素肺损伤模型,地塞米松组和贝母合剂组分别予地塞米松、贝母合剂治疗。用免疫组化SP法检测肺组织AQP1、ICAM-1和VCAM-1,并采用光学显微镜和Image Pro Plus6.0测量计算其平均光密度(IOD)值。结果:模型组大鼠肺组织AQP1表达显著低于假手术组(P<0.01),而ICAM-1、VCAM-1表达显著高于假手术组(P<0.01)。与模型组相比,贝母合剂组肺组织AQP1表达明显升高(P<0.01),ICAM-1、VCAM-1表达明显降低(P<0.01);地塞米松组肺组织ICAM-1、VCAM-1表达低于模型组(P<0.01,P<0.05),而AQP1表达与模型组无显著性差异(P<0.05)。结论:促进肺损伤大鼠肺组织AQP1表达和抑制ICAM-1、VCAM-1表达可能是贝母合剂防治肺损伤的作用机制之一。

Objective：To investigate the intervention effect of Fritillaria Mixture on endothelial function in rats with PYM-induced lung injury.Methods：40 rats were randomly divided into sham operation group,model group,dexamethasone group and Fritillaria Mixture group and then injected BLM 5 mg / kg by intratracheal way to copy lung injury model.Dexamethasone group was treated with dexamethasone distilled in water and Fritillaria Mixture group was treated with Fritillaria Mixture.The expressions of AQP1,ICAM-1 and VCAM-1 in lung tissue were tested by immunohistochemistry SP method.The average optical density（IOD） of AQP1,ICAM-1 and VCAM-1 were calculated by optical microscopy and Image Pro Plus6.0.Results：The degree of AQP1 in lung tissue of model group rats was significantly lower than that of sham operated group rats（P〈0.01） but the degree of ICAM-1 and VCAM-1 was significantly higher（P〈0.01）.Compared with model group,the degree of AQP1 in lung tissue of Fritillaria Mixture group was significantly higher（P〈0.01） and the degree of ICAM-1 and VCAM-1 was significantly lower（P〈0.01）.The degree of ICAM-1 and VCAM-1 in lung tissue was lower than that of model group rats（P〈0.01,P〈0.05） while the degree of AQP1 showed no difference（P〈0.05）.Conclusion：To stimulate the expression of AQP1 and inhibit the expression of ICAM-1 and VCAM-1 in lung tissue of rats with BLM-induced lung injury is probably one of the mechanisms that Fritillaria Mixture treats BLM-induced lung injury.