摘要
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)是一种软电离质谱,适用于分析蛋白质等生物大分子物质,可用来绘制微生物的蛋白质图谱,达到鉴定的目的。基于MALDI-TOF MS的蛋白质谱技术具有快速、准确、敏感性高、高通量等特点,目前已应用于细菌和真菌的鉴定,其在微生物耐药性分析、分型和毒力研究等科研领域的应用也初有成效,尚有巨大的发展空间。本文就以MALDI-TOF MS为代表的蛋白质谱技术在微生物检验和科研中的应用作一综述。
Matrix-assisted laser desorption / ionization-time of flight mass spectrometry（ MALDI-TOF MS） is one kind of soft ionization mass spectrometry,which is suitable for the analysis of bio-macromolecule,such as protein. It can be used for microbial identification by mapping the microbial protein mass spectra. Since MALDI-TOF MS technology is well-known for its rapidity,accuracy,high sensitivity and high throughput,it has been applied in bacterium and fungus identifications. MALDI-TOF MS also has a good performance in the field of scientific research,including resistance analysis,bacterium typing and virulence study,and there is still a large space for development. In this paper,we reviewed the application of MALDI-TOF MS technology in microbial identification and scientific research.
出处
《检验医学》
CAS
2015年第2期102-107,共6页
Laboratory Medicine
基金
国家自然基金资助项目(81322025
81171623
81371875)
上海市卫生系统优秀青年人才计划(XYQ2011039)
上海市曙光人才计划项目(12SG03)
关键词
基质辅助激光解吸电离飞行时间质谱
微生物
检验
科研
Matrix-assisted laser desorption / ionization-time of flight mass spectrometry
Microbiology
Determination
Scientific research
