Microflex^(TM) MALDI-TOF MS和Vitek 2 Compact全自动微生物分析系统对肠杆菌科细菌鉴定能力的比较

Comparison on the identification abilities of Microflex^(TM) MALDI-TOF MS and Vitek 2 Compact automatic microbial analysis system for Enterobacteriaceae

目的比较MicroflexTM基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)和Vitek 2 Compact全自动微生物分析系统(简称Vitek 2 Compact)对肠杆菌科细菌的鉴定能力。方法采用MicroflexTMMALDI-TOF MS和Vitek 2 Compact同时对545株肠杆菌科质控菌株和临床分离菌株进行鉴定,鉴定结果不一致者采用沙门菌血清凝集试验或细菌16S r DNA基因测序予以确证。结果 MicroflexTMMALDI-TOF MS对545株细菌的种和属的鉴定率分别为97.1%、2.9%。Vitek 2 Compact对545株细菌的种、群、属的鉴定率分别为83.3%、13.9%和2.2%,鉴定错误率为0.2%,未鉴定率为0.4%。结论 MicroflexTMMALDI-TOF MS对肠杆菌科细菌的鉴定符合率高于Vitek 2 Compact,且操作快速、简便,成本低,可用于临床肠杆菌科细菌的常规快速鉴定。

Objective To compare the identification abilities of Microflex^TM matrix-assisted laser desorption /ionization-time of flight mass spectrometry（ MALDI-TOF MS） and Vitek 2 Compact automatic microbial analysis system（ Vitek 2 Compact） for Enterobacteriaceae. Methods A total of 545 isolates of Enterobacteriaceae from a variety of clinical and quality control isolates were identified by MicroflexTMMALDI-TOF MS and Vitek 2 Compact. The isolates were confirmed by 16 S r DNA gene sequencing,and Salmonella was identified by serum agglutination test,if the identification results of the 2 systems didn＇t match. Results The coincidence rates of 545 isolates of Enterobacteriaceae identified by Microflex^TM MALDI-TOF MS were 97. 1% to the species level and 2. 9% to the genus level,respectively.The coincidence rates of 545 isolates of Enterobacteriaceae identified by Vitek 2 Compact were 83. 3%,13. 9% and2. 2% to the species,group and genus levels,respectively. The false rate was 0. 2%,and the un-identification rate was0. 4%. Conclusions The identification coincidence rate of Enterobacteriaceae by Microflex^TM MALDI-TOF MS is higher than that of Vitek 2 Compact. Using MicroflexTMMALDI-TOF MS to identify Enterobacteriaceae is fast,simple to operate and low-cost,and it can be used in routine rapid identification of Enterobacteriaceae in clinical microbiology laboratories.