白介素13受体α2在哮喘小鼠肺组织细胞内表达与气道重塑的关系

Relationship between interleukin-13 receptor α2 expression and airway remodeling in asthmatic mice

目的探讨白介素13受体α2(IL-13Rα2)与气道重塑的的关系。方法用OVA腹腔注射致敏SPF级BALB/c小鼠,雾化吸入激发制作哮喘模型,同时予地塞米松腹腔注射干预,最后一次致敏后24 h处死小鼠,观察并测量肺组织形态学改变,用RT-PCR和免疫组化法分别检测小鼠肺组织中IL-13Rα2 mRNA和蛋白的表达及TGF-β1 mRNA表达。结果哮喘小鼠与对照组、地塞米松组小鼠比较,支气管管壁(17.58±0.78)vs.(12.61±0.87)vs.(13.89±0.94)、平滑肌面积(6.71±0.83)vs.(4.61±0.46)vs.(5.62±0.53)均明显增加,P<0.01,RT-PCR发现肺组织中IL-13Rα2 mRNA(0.670±0.183)vs.(0.075±0.058)vs.(0.262±0.138)和TGF-β1 mRNA(0.704±0.168)vs.(0.317±0.140)vs.(0.358±0.098)明显增高,P<0.01,免疫组化发现IL-13Rα2蛋白表达(52.11±6.30)vs.(17.80±4.09)vs.(22.87±3.76)明显增高,P<0.01。结论 IL-13Rα2在哮喘小鼠肺组织表达增加,与气道重塑密切相关。

Objective To investigate the relationship between interleukin-13 receptor α2（IL-13Rα2） and airway remodeling. Methods SPF level BALB/c mice were injected with OVA intraperitoneal by sensitization, and inhalation stimulation, and dexamethasone intervention by intraperitoneal injection. Mice were sacrificed 24 h after the last sensitization. It was observed that morphological changes in mice lung tissue. IL-13 Rαt2 mRNA , TGF-β1 mRNA and IL-13 Rα2 protein expression in mice lung tissues were detected by reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemisty respectively. Results Compared with the control group, dexamethasone group, the bronchial wall of asthmatic mice became thicker （ 17.58 ± 0.78） vs. （ 12.61± 0.87） vs. （ 13.89 ±0.94）, P 〈 0.01, and their smooth muscle area was much thicker too （6.71 ±0.83） vs. （4.61 ±0.46） vs. （5.62 ±0.53）, P 〈0.01. RT-PCR found that IL-13R α2 mRNA （0. 670 ± 0. 183 ） vs. （0. 075 ± O. 058 ） vs. （0. 262±0. 138 ） and TGF-β1 mRNA （0. 704 ± 0. 168） vs. （0. 317 ± 0.140） vs. （0. 358 ± 0. 098） significantly increased in lung tissue, P 〈 0. 01, and immunohistochemistry showed that IL-13Rα 2 protein expression （52. 11 ± 6.30） vs. （ 17.80 ± 4.09） vs. （ 22.87 ±3.76 ） was evidently higher, P 〈 0.01. Conclusion The expression of IL-13 Rα2 in asthmatic mice lung tissue is increased, which has close relationship with airway remodeling.