同源导入近交系绿色荧光裸小鼠Foxn1^(nu).B6-CAG-EGFP/SU的建立

Establishment of a new congenic inbred mouse strain named Foxn1^(nu). B6-CAG-EGFP/SU

目的建立一个能稳定表达增强型绿色荧光蛋白(enhanced green fluorecence protein,EGFP)的裸小鼠近交新品系,供包括人脑胶质瘤在内的所有人类肿瘤移植实验用。方法将雌性C57BL/6-Tg(CAG-EGFP)转基因小鼠和雄性Foxn1nu裸小鼠,按同源导入方法进行杂交和回交,用荧光手电筒和匹配的眼镜、多功能活体成像仪、荧光显微镜对是否表达EGFP进行鉴别,传至F10后进行遗传学检测。结果所建品系命名为Foxn1nu.B6-CAGEGFP/SU(Soochow University),14个遗传生化位点中13个表型与BALB/c(Foxn1nu)吻合,唯Pep3(肽酶-3)的电泳为b型,而标准的BALB/c近交系为a型;外周血淋巴细胞占全部有核细胞15%,T淋巴细胞仅占0.3%,与亲本的Foxn1nu一致。结论成功地建立了一个既与亲本C57BL/6-Tg N(CAG-EGFP)-样稳定表达EGFP,又与Foxn1nu裸小鼠(BALB/c背景)一样具有T细胞缺乏的荧光裸小鼠新品系-Foxn1nu.B6-CAG-EGFP|SU,Pep3为b型是有别于Foxn1nu裸小鼠的生化遗传标记位点。

Objective To establish a new congenic inbred mouse strain carrying and expressing EGFP and Foxn1^nugene for cancer research including human glioma as well. Methods According to criterion of GB14923- 2010,the male Foxn1^nunude mice backcross the female C57 BL /6-Tg（ CAG-EGFP） transgenic mice for 10 times,Then identify the phenotype using the methods and equipment as below： fluorescent flashlight and matching glasses; multifunction vivo imager; fluorescence microscopy. Results The congenic inbred mouse strain named Foxn1^nu. B6-CAG-EGFP / SU（ Soochow University）. All the 14 biochemical loci are homozygous and same with Balb/c mouse in addition to the Pep3loci（ ＂b＂type instead of ＂a＂type）. Peripheral blood lymphocyte count shows the lymphocytes occupy 15% of nucleated cells; T lymphocytes occupy 0. 3%,meet the requirement of inbred strain of EGFP nude mice. Conclusions Established a new congenic inbred strain-Foxn1^nu. B6-CAG-EGFP / SU which both express EGFP stably,and own immunodeficiencywith lack of T lymphocytes. The phenotype＂b＂of biochemical loci＂Pep3＂is the unique characteristic that distinguish SU to Foxn1^nu.