P物质与香草酸瞬时受体亚型1在慢性非细菌性前列腺炎大鼠神经痛中的作用研究

Roles of substance P and transient receptor potential vanilloid 1 in neuralgia in rats with chronic nonbacterial prostatitis

目的：探讨慢性非细菌性前列腺炎（CNP）疼痛的可能机制。方法：自身免疫法建立CNP大鼠模型;Von Frey丝检测机械刺激缩足阈（PWT）;进行前列腺病理学检查和免疫组化检测前列腺与脊髓L5-S2节段P物质（SP）、香草酸瞬时受体亚型1（TRPV1）表达变化和相关性。结果：造模后大鼠PWT[（48.83±11.55）g]下降,与对照组[（67.98±5.44）g]相比差异有统计学意义（P〈0.05）。前列腺呈现显著炎症反应,病变范围、间质淋巴细胞浸润都较对照组反应程度有所加重,差异有统计学意义（P〈0.05）。与对照组相比,模型组前列腺与脊髓背角L5-S2中SP、TRPV1阳性表达均上调,差异有统计学意义（P〈0.05）。前列腺与脊髓中SP蛋白表达无相关关系（r=0.099,P=0.338）;TRPV1蛋白表达亦无相关关系（r=0.000,P=0.5）。结论：SP、TRPV1可能通过脊髓L5-S2节段的表达上调参与了CNP大鼠疼痛的形成和维持。

Objective： To study the possible mechanisms of chronic nonbacterial prostatitis （CNP） pain. Methods： CNP models were established in male Wistar rats by the autoimmune method. Then the paw withdrawal threshold （PWT） was detected using the Von Frey filament, prostate pathological examination was conducted, the expressions of substance P （SP） and transient receptor potential vanilloid 1 （ TRPV1 ） in the prostate tissue and 1.5 - S2 spinal segments were determined by immunohistochemistry and their correlations were analyzed. Results： Compared with the control group, the CNP model rats showed markedly decreased PWT （P 〈 0.05 ） and obvious inflammation in the prostate tissue, with significant differences in the scope of lesion and interstitial lymphocyte in- filtration （ P 〈 0.05 ）. The expressions of SP and TRPV1 in the prostate and spinal cord dorsal horn L5 - S2 were remarkably upregu- lated in the models as compared with the control rats （P 〈 0.05 ）. However, the expression of SP in the prostate was not correlated with that in the spinal cord （r = 0.099, P= 0.338）, nor was that ofTRPV1 （r = 0.000, P = 0.5）. Conclusion： SP and TR- PV1 were involved in the formation and persistence of pain in CNP rats through their upregulated expressions in the L5 - S2 spinal segments.