环氧合酶-2-1195G/A和锰超氧化物歧化酶9Ala/Val基因多态性与高脂饮食的交互作用及其与溃疡性结肠炎的关系

Interaction between the Polymorphisms of Cyclooxygenase-2-1195G / A,MnSOD9Ala / Val Genes and the High-fat Diets and Its Correlation with Ulcerative Colitis

目的探讨环氧合酶-2-1195G/A（COX-2-1195G/A）和锰超氧化物歧化酶9Ala/Val（Mn SOD9Ala/Val）基因多态性与高脂饮食的交互作用及其与溃疡性结肠炎（UC）的关系。方法采用病例-对照研究的方法,以750例UC患者及750例健康对照者的外周血白细胞为样本,采用聚合酶链反应（PCR）技术分析COX-2-1195G/A和Mn SOD9Ala/Val基因多态性。结果 UC组和对照组COX-2-1195G/A（A/A）基因型的分布频率分别为49.07%和21.20%,Mn SOD9Ala/Val（V/V）基因型的分布频率分别为50.13%和22.40%,差异均有统计学意义（P均〈0.01）。COX-2-1195G/A（A/A）基因型（OR=3.5808,95%CI=1.8062-5.3478）和Mn SOD9Ala/Val（V/V）基因型（OR=3.4828,95%CI=1.9137-5.5496）者患UC的风险均显著增加。基因突变的协同分析结果显示,COX-2-1195G/A（A/A）/Mn SOD9Ala/Val（V/V）基因型者在UC组和对照组中的分布频率分别为40.67%和8.40%,差异有统计学意义（P〈0.01）,COX-2-1195G/A（A/A）/Mn SOD9Ala/Val（V/V）基因型者患UC的风险显著增加（OR=7.5655,95%CI=4.1849-11.2037）。UC组高脂饮食率显著高于对照组（49.73%比20.13%,P〈0.01）,高脂饮食与COX-2-1195G/A（A/A）（γ=11.81821）和Mn SOD9Ala/Val（V/V）（γ=9.0107）基因型均有交互作用。结论 COX-2-1195G/A（A/A）和MnSOD9Ala/Val（V/V）基因型及高脂饮食是UC的易患因素,基因多态性与高脂饮食的交互作用增加了UC的发病风险。

Objective To investigate the interaction of the polymorphisms of cyclooxygenase-2-1195 G / A（ COX-2-1195 G / A） and manganese superoxide dismutase 9 Ala / Val（ Mn SOD9 Ala / Val） genes and the high-fat diets and its potential correlation with ulcerative colitis（ UC）. Methods The genetic polymorphisms of COX-2-1195 G / A and Mn SOD9 Ala / Val were analyzed by polymorphism-polymerase chain reac-tion（ PCR） in peripheral blood leukocytes obtained from 750 UC patients（ UC group） and 750 healthy subjects（ control group）. Results The frequencies of COX-2-1195 G / A（ A / A） and Mn SOD9 Ala / Val（ V /V） were 49. 07 % and 50. 13 % in UC group and 21. 20 % and 22. 40 % in control group,respectively（ P〈0. 01）. The risk of UC significantly increased in subjects with COX-2-1195 G / A（ A / A） genotype（ OR =3. 5808,95 % CI = 1. 8062-5. 3478） and in those with Mn SOD9 Ala / Val（ V / V） genotype（ OR = 3. 4828,95 % CI = 1. 9137-5. 5496）. Pooled analysis of the polymorphisms showed that distribution frequency of COX-2-1195 G / A（ A / A） / Mn SOD9 Ala / Val（ V / V） was 40. 67 % in UC group and 8. 40 % in control group（ P〈0. 01）. Subjects with COX-2-1195 G /A（ A /A） /Mn SOD9 Ala /Val（ V /V） had a significantly higher risk of UC（ OR = 7. 5655,95 % CI = 4. 1849-11. 2037）. The rate of high-fat diets was significantly higher in the UC group than in the control group（ 49. 73 vs. 20. 13 %,P 0. 01）,and statistic analysis suggested an interaction between high-fat diet and COX-2-1195 G / A（ A / A）（ γ = 11. 81821） and Mn SOD9 Ala / Val（ V /V）（ γ = 9. 0107）,which increase risk of UC. Conclusions COX-2-1195 G /A（ A /A）,Mn SOD9 Ala /Val（ V / V）,and high-fat diet are the risk factors of UC. The interaction between the genetic polymorphisms of COX-2-1195 G / A and Mn SOD9 Ala / Val and the high-fat diet increases the risk of UC.