黄药子醇提物抑制胃癌细胞功能的研究

Inhibiting effect of alcohol extract from Dioscore bulbifera on gastric cancer cells

目的研究黄药子醇提物对人胃癌细胞增殖、克隆形成和迁移能力的影响。方法制备黄药子醇提物,根据细胞培养液所含黄药子醇提物的不同浓度,将实验分为黄药子醇提物高浓度组（120 mg/L）、低浓度组（60 mg/L）和对照组（等量无菌水）。通过体外MTT、克隆形成实验、细胞迁移实验,分别检测黄药子醇提物对人胃癌细胞系MGC803增殖、克隆形成和迁移能力的影响。结果与对照组相比,黄药子醇提物高浓度组和低浓度组胃癌细胞体外增殖速度明显减慢（培养第2-6天时,F分别为29.130、21.864、67.826、36.015、43.656,均P〈0.01）、平板克隆形成率明显减少（F=11.918,P〈0.01）,黄药子醇提物高浓度组的细胞迁移能力明显降低（F=4.258,P〈0.05）。结论黄药子醇提物可以显著抑制胃癌细胞增殖、克隆形成和迁移能力,具有潜在的抑制胃癌细胞转移的能力。

Objective To investigate the inhibiting effects of alcohol extract from Dioscore bulbifera on proliferation,colony formation and migration of cancer cell lines. Methods Alcohol extract from Dioscore bulbifera was prepared usingSoxhlet extraction. Human gastric cancer cell line MGC803 was treated with different concentrations（0, 60, 120 mg/L）of alcohol extract from Dioscore bulbifera. In vitro, proliferation, colony formation and migration of gastric cancer cells were detect-ed by MTT, colony formation experiments and Transwell assay respectively. Results The proliferation（day2-day 6, F=29.130, 21.864, 67.826, 36.015, 43.656, P〈0.01）and colony formation（F=11.918,P〈0.01）of gastric cancer cells weresignificantly inhibited by administration of alcohol extract from Dioscore bulbifera at both 60 mg/L and 120 mg/L. The migra-tion（F=4.258,P〈0.05）of gastric cancer cells were significantly suppressed after cells were treated with120 mg/L alcohol ex-tract from Dioscore bulbifera. Conclusion Alcohol extract from Dioscore bulbifera significantly inhibit proliferation, colonyformation and migration of gastric cancer cells.