电针刺激对Beagle犬展神经损伤修复的研究

Effect of electroacupuncture on abducens nerve injury in Beagle dog

目的建立展神经损伤电针刺激的动物模型,研究电针刺激对展神经损伤修复的疗效。方法 24只Beagle犬随机均分为单纯损伤组（对照组）及损伤后电针刺激组（实验组）。行展神经脑池段压榨损伤,展神经刺激电极及外直肌记录电极植入。于术后1-12周,测量2组瞳孔中心至外眦内侧缘距离。结果所有Beagle犬均能耐受手术,颅内刺激电极和外直肌记录电极成功植入;术后1-2周,2组Beagle犬瞳孔中心至外眦内侧缘距离比较差异无统计学意义,术后4-12周,实验组Beagle犬瞳孔中心至外眦内侧缘距离小于对照组（P〈0.01）。结论成功建立应用于展神经损伤电针刺激的动物模型,电针刺激可以明显促进损伤的展神经恢复。

Objective To establish Beagle dogs＇ model of abducens nerves injury and to observe the clinical therapeu-tic effect of electroacupuncture treatment. Methods Twenty-four Beagle dogs were randomly divided into simple crushgroup（control group） and crush with electrical stimulation group（experimental group）. Cisternal segment of the abducensnerve was given a crush injury, then electrodes were implanted to stimulate the abducens nerve and lateral rectus muscle.Distance between the center of the pupil to medial margin of extraocular adjoin was measured from 1 to 12 weeks after opera-tions. Results All procedures used in the study were well tolerated by Beagle dogs. Electrode implantation to stimulate thelateral rectus muscle and the abducens nerve behind of cavemous sinus was successful. There was no statistical significanceof the distance between the two groups from 1 to 2 weeks after operations, and the distance was shorter in experimental groupthan that in control group from 4 to 12 weeks after operations（P 〈0.01）. Conclusion The animal models established tostudy electroacupuncture treatment of the injured abducens nerves was successful. Electroacupuncture can promote the recovery of the injured abducens nerves obviously.