高产蛋白酶枯草芽孢杆菌JNB001产酶条件的优化

Optimization of Fermentation Conditions on the Protease-producing Bacillus subtilis JNB001

为了进一步提高枯草芽孢杆菌JNB001产蛋白酶的能力,试验利用单因素及正交试验从起始pH、发酵温度、接种量、装液量、菌龄等方面对产酶条件进行优化。结果表明,最佳培养条件为:起始pH 7.0;发酵温度35℃;接种量7%;装液量35mL/250mL;菌龄18h;发酵时间36h。菌株JNB001经优化发酵条件后,测定其蛋白酶活力可高达371.66U/mL。该试验结果为无害化生物处理罐的优化设计等后续应用研究奠定了基础。

This research was aimed to further improve the ability of protease produced by Bacillus subtilis JNB001. By series single factor and orthogonal analysis to optimize the enzyme-producing conditions from initial pH,fermentation temperature,inoculation volume, bottling volume and cell age, etc. The results showed that the optimum conditions were：Initial pH 7.0,inoculation volume of 7G,bottling volume of 35 mL in the 250 mL flask,cell age was 18 h,and cultured for 36 h at 35 ~C. Cultivated at these conditions, the protease activity of the strain JNB001 could reach to 371.66 U/mL. These consequences laid the foundation for the subsequent applications such as the design of harmless biological treatment tank, etc.