一种简易同时蒸馏萃取方法用于八角茴香挥发油提取及其GC-MS分析

A Simple Simultaneous Distillation/Solvent Extraction Method Coupled with Gas Chromatography-Mass Spectrometry for the Analysis of Volatile Oils of Illicium Verum

设计了一种简易同时蒸馏萃取(SSDE)装置并用于八角茴香挥发油提取及其气相色谱-质谱法(GCMS)分析。该装置由圆底烧瓶、恒压滴液漏斗和回流冷凝管构成。通过对八角茴香挥发油组分的GC-MS分析,比较SSDE法和水蒸汽蒸馏提取法(HD)提取植物源挥发油的效果。结果表明,SSDE法所得挥发油相对含量在0.1%以上的19种化合物占挥发油总量的98.84%(相对标准偏差(RSD)(n=3)均不大于5.59%,其中RSD﹤5%的17种化合物占鉴定组分89.5%。SSDE法和HD法挥发油平均收率分别为7.30%(RSD=2.37%(n=5))和6.67%(RSD=5.26%(n=5))。SSDE法简便、快速,适用于八角茴香挥发油的提取。

A simple simultaneous distillation and solvent extraction （SSDE） method was developed and had been applied for sample preparation of the gas chromatography-mass spectrometry（GC-MS） analysis of volatile components from Illieium Verum. The simple apparatus was composed of a round flask, a constant pressure dropping funnel and a condenser. The constituents of the volatile oil obtained by SSDE were compared with those obtained by hydrodistillation method（HD） followed by GC-MS analysis. In terms of GC-MS analysis of volatile oil, the relative standard deviation （RSD） values （n = 3 ） of measured relative contents （ 〉 0. 1% ） of 19 identified compounds are no more than 5.59%. The total content of above components is 98.84% of the volatile oil, in which there are 17 components in 89.5% content with RSD less than 5%. And the yield of volatile oil obtained by SSDE and HD is 7.30% （RSD 2.37% （n =5） ） and 6.67% （RSD 5.26% （n =5） ）, respectively. These results indicate that SSDE is a simple, suitable method for the extraction of volatile oil from Illicium Verum.