五味子中非法添加苋菜红、日落黄、亮蓝的检测

Detection of Pigments Added Illegally in Schisandrae Chinensis Fructus

目的建立检测五味子中非法添加色素苋菜红、日落黄、亮蓝的方法。方法采用高效液相色谱法-二极管阵列检测器（HPLC-PDA）法初步鉴定五味子中非法添加色素苋菜红、日落黄、亮蓝,并测定3种色素的含量。结果苋菜红、日落黄、亮蓝的最低检出限为2.0~5.0 ng,线性范围为0.025~0.75μg,加样回收率为94.43%~100.58%,相对标准偏差（RSD）均小于2.0%。结论此法简单、准确,可作为检测五味子中非法添加色素苋菜红、日落黄、亮蓝的有效方法。

Objective To develop a method for the detection of amaranth, sunset yellow and brilliant blue illegally added into Schisandrae Chinensis Fructus. Methods HPLC-PDA was used for the preliminary identification and content determination of pigments mentioned above. Results For amaranth, sunset yellow and brilliant blue, the detection limits were in the range of 2.0-5.0 ng, the calibration curves showed good linearity in the range of 0.025-0.75 μg, the recoveries were from 94.43 % to 100.58 %, and the RSDs were lower than 2.0 %. Conclusion The method is simple and accurate, which can be used for the detection of pigments illegally added into Schisandrae Chinensis Fructus.