银杏叶聚戊烯醇纳米乳液的制备研究

Preparation of Polyprenols Nanoemulsion from Ginkgo biloba L.Leaves Polyprenols

以银杏叶聚戊烯醇为原料,Span-80与Tween-80为乳化剂,采用反相乳化(EIP)法,制备聚戊烯醇的水包油型纳米乳液,分别对影响聚戊烯醇纳米乳液性能的乳化剂的亲水疏水平衡(HLB)值、乳化剂与聚戊烯醇的比例、搅拌时间、乳化温度和搅拌速度等因素进行单因素考察。并对搅拌时间、乳化温度和搅拌速度进行3因素3水平的Box-Behnken响应面分析。结果显示:当乳化剂HLB值为9.5,乳化剂与聚戊烯醇质量比为1.75∶1,搅拌时间为12 min,乳化温度为60℃以及搅拌速度为17 000 r/min时,聚戊烯醇纳米乳液平均粒径最小,为97 nm,与模型模拟值(92 nm)的RSD为2.7%(<5%),粒径分布系数为0.383,说明该模型模拟得到的最佳工艺参数对实际操作的预测较为可靠,具有一定的指导意义。优化后的聚戊烯醇纳米乳液的离心稳定性、贮藏稳定性、分散性以及冻融后分散性均达到1级,乳液呈现出良好的均匀性;而冻融后离心乳液会初现稠度不均的情况,即2级水平,并保持该状态。

The preparation of oil-in-water type polyprenols nanoemulsion from Ginkgo biloba L. leaves polyprenols with inversed phase emulsification（EIP） method was studied. On the basis of single factor,the effects of factors, such as hydrophile-lipophile balance（HLB） number, proportion of emulsifier to polyprenol, mixing time, emulsionizing temperature, and mixing rate, on the preparation process and average diameter of polyprenols nanoemulsion were investigated. The preparation conditions,i, e. , mixing time, emulsionizing temperature, and mixing rate,were also optimized by using response surface method （RSM）. Results showed that the optimum conditions were mixing time 12 min, emulsionizing temperature 60 ~C, and mixing rate 17 000 r/min. Under these conditions, the average diameter of polyprenols nanoemulsion was the minimum and the RSD of the actual value （97 nm） with the fitted value （92 nm） was 2.7% （ 〈 5% ）, and the distribution coefficient of particle diameters was 0.383. The optimal parameters obtained by simulating this model were reliable in predicting the actual operation, and had certain guiding significance. The centrifugal stability, storage stability, diepersity, and freezing-thawing dispersity of polyprenol nanoemulsion under the optimum conditions showed good uniformity and reached l-level. The freezing-thawing centrifugation stability of polyprenol nanoemulsion showed preliminary visible uniformity, which was 2-level, and kept 2-level.