维生素C对实验兔硝酸甘油耐受性影响的研究

The effect of vitamin C on nitroglycerin tolerance

目的探讨维生素C对硝酸甘油耐受性的影响。方法选取30只兔龄5~6个月的健康家兔,将取出的兔胸主动脉环按随机数字表法分为3组：对照组、硝酸甘油组、硝酸甘油＋维生素C组,每组10份,分别做硝酸甘油量效关系及超氧化物歧化酶（SOD）活力和丙二醛（MDA）水平测定。结果对照组的最大舒张程度为（96±7）%,硝酸甘油组的最大舒张程度为（63±7）%,硝酸甘油＋维生素C组的最大舒张程度为（86±5）%,硝酸甘油组、硝酸甘油＋维生素C组可以明显增强兔离体动脉环对硝酸甘油的反应性。硝酸甘油组血管组织中MDA含量明显要高于对照组和硝酸甘油＋维生素C组,差异有统计学意义（P〈0.01）,而对照组和硝酸甘油＋维生素C组的MDA含量差异无统计学意义。硝酸甘油组血管组织中SOD活力明显要低于对照组和硝酸甘油＋维生素C组,差异有统计学意义（P〈0.05）,而对照组和硝酸甘油＋维生素C组的SOD活力差异无统计学意义。结论维生素C可改善硝酸甘油的耐受性。

Objective To study the effect of vitamin C on tolerance to nitroglycerin.Methods Thirty cases of normal rabbits aged 5-6months were divided into three groups randomly,including control group,nitroglycerin group and nitroglycerin ＋ vitamin C group.Effect relationship of nitroglycerin,SOD activity and MDA in tissues were determined at the end of the experiment.Results The maximum diastolic level,SOD activity and MDA in control group were（96±7）%,（388±42）U/mg,（1.66±0.09）nmol/mg,and those of nitroglycerin group were（63±7）%,（330±28）U/mg,（9.44±0.63）nmol/mg,while in nitroglycerin＋vitamin C group were（86±5）%,（378±39）U/mg,（1.71±0.16）nmol/mg,respectively.The maximum diastolic level,SOD activity in control group were higher than those of nitroglycerin group,the level of MDA in control group were lower than that of nitroglycerin group.The maximum diastolic level,SOD activity in nitroglycerin＋ vitamin C group were higher than those of nitroglycerin group,the level of MDA in nitroglycerin ＋ vitamin C group were lower than that of nitroglycerin group.Conclusion Vitamin C can prevent or attenuate the development of the tolerance.