枯草芽孢杆菌抗菌物质初步探究

Primary Research of Bacillus subtilis on Antimicrobial Substance

以金黄色葡萄球菌为指示菌，对发酵液进行一系列处理，初步探究抗菌物质的性质。将上清液在不同温度下处理，40-70℃处理时，抑菌性没有明显变化，80℃以后抑菌性随着处理温度的升高而逐渐降低，121℃处理后，抑菌活性几乎消失。酸碱和有机溶剂处理后，上清液的抑菌活性有所下降，但仍保持一定的抑菌活性，处理时有白色沉淀产生。上清液对蛋白酶都比较敏感，用胃蛋白酶、胰蛋白酶、木瓜蛋白酶处理1h时，抑菌活性几乎消失。通过以上结果，可以初步判定枯草芽孢杆菌YB5能够产生蛋白类抑菌物质。

Staphylococcus aureus as the indicator bacteria, curing fermentation liquor, re- searched character of antimicrobial substance. Putting liquid supernatant in different temperature fond that 40℃ to 70℃ processing, the antibacterial activity did not change significantly ;80℃ pro- cessing ,the antibacterial activity was gradually decreased with the rise of temperature;after 121 processing, the antibacterial activity was almost disappeared. The antibacterial activity declined af- ter acid base and organic solvent processing, but it was still keep a certain antibacterial activity and pruduced white precipitation in the processing. Liquid supernatant was sensitive about prote- ase when using pepsin, trypsin and papain processed 1 h , the antibacterial activity were almost disappeared. The above results estimated that Bacillus subtilis YB5 could produce antimicrobial substance which belonged to protein.