摘要
通过对分化培养基和生根培养基进行优化,完善了宝坻大蒜再生体系,确定最佳分化培养基为MS+6-BA 5.0 g/L+NAA 1.0 g/L,最佳生根培养基为无激素的MS培养基。对再生植株进行细胞学鉴定及田间评估,未发现染色体变异,再生植株田间特性有利于提高光合效率。
The regeneration system of Baodi garlic was improved by optimizing the differentiation medium and rooting medium. The optimal differentiation medium was MS＋6-BA 5.0 g/L＋NAA 1.0 g/L and the best medium for rooting was MS medium without hormones. No chromosomal aberration was observed according to the results of the cytological identification and field assessment of the plant regeneration. The field features indicated the improvement of the photosynthetic efficiency of the plant regeneration.
出处
《生物技术通报》
CAS
CSCD
北大核心
2015年第2期97-102,共6页
Biotechnology Bulletin
基金
天津市农委重点项目(5KN13001)
天津师范大学应用开发研究基金项目
天津市科委面上项目(14JCYBJC29900)
关键词
宝坻大蒜
再生植株
叶夹角
鳞茎
Baodi garlic
plant regeneration
leaf angle
bulb
