电致化学发光法高灵敏度测定血清中的辛可宁

Highly Sensitive Determination of Cinchonine in Serum Samples by Electrochemical Luminescence Method

利用金电极为工作电极,研究了辛可宁-Ru（bpy）3^2＋体系的电致化学发光行为。研究表明,辛可宁对Ru（bpy）3^2＋在电极表面的电致化学发光具有显著的增强作用,据此建立了一种高灵敏度测定辛可宁的电致化学发光分析方法。考察了硼酸-硼砂缓冲溶液的浓度、p H值、联吡啶钌浓度、光电倍增管电压等参数对实验的影响。在12 mmol/L硼酸-硼砂缓冲溶液（p H 9.0）中,辛可宁浓度的对数lgc在3.0×10^-9-6.0×10^-6mol/L范围内与Ru（bpy）2＋3的电致化学发光强度变化值（ΔI）呈线性关系,检出限（S/N=3）为1.76×10^-10mol/L。应用此法对血清中辛可宁的浓度进行测试,加标回收率为102.1%-109.5%。

In this paper, the eletrochemical luminescence（ ECL） behaviors of cinchonine- Ru（ bpy）3^2 ＋ system was studied with the gold electrode as working electrode. ECL intensity of Ru（ bpy）3^2 ＋ could be enhanced significantly in the presence of cinchonine. Based on this fact,a high sensitivity ECL method was established for the determination of cinchonine at gold electrode. Some detection conditions such as concentration and p H value of buffer solution, concentration of Ru（ bpy）2 ＋3and voltage of photomultiplier tube were optimized. In 12 mmol / L boric acid- borax buffer solution（ p H 9. 0）,the ECL peak counts increased linearly with concentration of cinchonine in the range of 3. 0 × 10^- 9- 6. 0 × 10^- 6mol / L. The detection limit（ S / N = 3） for cinchonine was 1. 76 ×10^- 10 mol / L. The method was applied in the determination of cinchonine in serum with recoveries of 102. 1%- 109. 5%.