微波等离子体发射光谱法同时测定特色南药中多种元素

Simultaneous Determination of Multiple Elements in Characteristic South China Herbal Medicine by Microwave Plasma Atomic Emission Spectrometry

特色南药样品利用硝酸微波消解后,在优化的还原剂浓度下,采用多模式样品导入系统和微波等离子体原子发射光谱法（MP-AES）对样品溶液中Al,As,Hg,Ca,Cu,Cr,Pb,Zn等17种元素进行同时分析,考察了其在特色南药材中的含量。结果表明,MP-AES对目标元素有较好的检出限,2种标准物质的分析结果与ICP-MS分析结果及标准值一致,其中可蒸气发生元素（As,Hg,Sb和Se等）未检出。方法对金属元素的检出限为0.05-0.53μg/g,对于氢化物可蒸气发生元素和Fe的检出限为0.93-5.40μg/g。通过该方法进行测定,发现南药样品中Cd和Cu含量低于中国药典的限值,而Cr和Pb含量明显高于欧洲草药材的限值,As和Hg需优化方法降低检出限后方可用于样品质量控制。本方法检出限较低,线性范围较宽,分析结果准确、快速,可进行多元素同时分析,能满足对南药材中多种元素的分析要求。

Characteristic south China herbal medicine samples were digested by microwave system with nitric acid,and 17 kinds of elements,such as Al,As,Hg,Ca,Cu,Cr,Pb and Zn,etc in the digestion solutions were simultaneously analyzed by microwave plasma atomic emission spectrometry（ MP- AES） with multi-mode sample introduction system（ MSIS） under the optimized reductant concentration,to investigate the contents of these elements in south herbal medicines. Result showed that MP- AES had good detection limits for metal elements,and the analytical results of elements in two kinds of standard material were consistent with the analytical results of ICP- MS and the standard values,except for As,Hg,Sb and Se that were undetected. The detection limits of the method were 0. 05- 0. 53 μg / g for the metal elements, and 0. 93- 5. 40 μg / g for elements that could generate the vapor with hydride. In these south China herbal medicine samples,concentrations of Cd and Cu were below the limit values of Chinese Pharmacopoeia,contents of Cr and Pb exceeded the limits of European herbs,but the detection limits of As and Hg needed to decrease for quality control of herbs. The method could meet the analysis requirements of some elements in the south China herbal medicine because of its low detection limit,wide linear range,accuracy,rapid analysis and simultaneous multi-element analysis.