骨质疏松肾阳虚病证结合模型构建的实验研究

Study on Osteoporosis Mice Model with Syndrome of Deficiency of Kidney-Yang

目的研究建立骨质疏松肾阳虚病证结合模型的方法。方法雌性昆明小鼠随机分为对照组(A组)、证型模型组(B组)、疾病模型组(C组)、疾病证型模型组(D组)。分别应用去双侧卵巢法构建骨质疏松模型,氢化可的松皮下注射诱导肾阳虚证模型。通过观察小鼠症状体征、促肾上腺皮质激素、促甲状腺激素、环磷酸腺苷、环磷酸鸟苷、骨密度、骨组织形态学等评价模型。结果与A组比较,D组小鼠出现毛发干枯,畏寒倦卧,肛温降低等肾阳虚症状,促肾上腺皮质激素及促甲状腺激素含量降低(P<0.01),环磷酸腺苷/环磷酸鸟苷降低(P<0.01),股骨近端、腰椎骨密度下降(P<0.01),HE染色及骨组织形态计量学分析显示骨小梁排列稀疏、变细,破骨细胞数增多等。结论采取双侧卵巢切除结合氢化可的松皮下注射的方法,能成功构建骨质疏松肾阳虚病证结合模型。

Objective： To establish the osteoporosis model with syndrome of deficiency of Kidney-Yang. Methods： Female Kunming mice were randomly divided into control group（group A）,syndrome group（group B）,disease group（group C）,disease with syndrome group（group D）. The model was established by injecting hydrocortisone and resecting bilateral ovaries. Signs and symptoms,level of adrenocorticotropic hormone（ACTH）,thyroid stimulating hormone（TSH）,cyclic adenosine monophosphate（c AMP）,cyclic guanosine phosphate（c GMP）,bone density bone,and tissue morphology were observed to evaluate the model. Results： Compared with group A,mice in model group appeared obvious kidney-Yang deficiency symptoms,including hair dry,chill and frail state,low anal temperature. The levels of ACTH and TSH decreased（P 〈0.01）,and the ratio of c AMP/c GMP decreased（P 〈0.01）. Bone density of lumbar vertebra and femur decreased significantly（P 〈0.01）. HE staining revealed osteoporosis of mice in group D. Conclusion： The model of osteoporosis with type of deficiency of Kidney-Yang can be established successfully by injecting hydrocortisone and resecting bilateral ovaries.