CdSe/ZnS量子点对斑马鱼胚胎发育的影响

The effects of CdSe/ZnS quantum dots on embryonic development of zebrafish

目的 研究CdSe/ZnS量子点对斑马鱼胚胎发育的影响。方法 以0、0.5、1、2、4、8和16nmol/LCdSe/ZnS量子点分别处理斑马鱼胚胎,于受精后24、48、72、96和120 h（简称hpf）5个时间点分别观察各自具有代表性的毒理学终点。结果 在120hpf时,CdSe/ZnS量子点对斑马鱼胚胎的LC50为21.38nmol/L（95%CI：17.21～26.57）。量子点CdSe/ZnS对斑马鱼胚胎24hpf时60s内胚胎自主运动频率,48hpf60s内心率、胚胎孵化率、死亡率等均有明显影响,高浓度8nmol/L和16nmol/L组CdSe/ZnS量子点在120hpf时可致斑马鱼胚胎发生心包水肿、肝脏变小、卵黄囊吸收延迟、肠道发育异常及肌肉变性等中毒症状。结论 8nmol/L及以上浓度CdSe/ZnS量子点对斑马鱼胚胎具有较强的发育毒性,暴露浓度和时间的增加,CdSe/ZnS量子点可造斑马鱼胚胎死亡率升高。

Objective To understand the developmental effects induced by CdSe/ZnS quantum dots（ QDs） on zebrafish embryos. Methods Zebrafish embryos were exposed to 0,0. 5,1,2,4,8 and 16 nmol / L of CdSe/ZnS QDs,and the typical toxicological indexes were recorded at five time points respectively（ 24 hours post fertilization（ hpf）,48 hpf,72 hpf,96 hpf,120 hpf）. Results The results showed that the median lethal concentration（ LC50） for zebrafish embryos after 120 hpf was 21. 38 nmol / L（ 95% CI = 17. 21- 26. 57）. The frequency of spontaneous movement in 60 seconds after24 hpf,the frequency of heart beat in 60 seconds after 48 hpf,the hatching rate and the mortality rate were obviously affected by CdSe/ZnS QDs. Several abnormalities and toxic symptoms caused by CdSe/ZnS QDs at 8 nmol / L and16 nmol / L were observed including pericardial edema,liver atrophy,non- depleted yolk,intestinal abnormal development and muscle degeneration after 120 hpf. Conclusion High level of CdSe/ZnS QDs（ more than 8 nmol / L） could induce toxic effects on zebrafish embryonic development.