脂肪细胞因子Chemerin对冠心病的预警作用

Early Warning of Chemerin Level in the Serum of Patients with CHD

目的:探讨脂肪细胞因子Chemerin在冠心病(CHD)发生与发展中的临床价值。方法:将20只健康雄性apo E-/-小鼠随机分为普食喂养组(NF组)10只和高脂喂养组(HF组)10只,实时荧光定量PCR(QPCR)检测2组小鼠腹主动脉旁脂肪中Chemerin mRNA相对表达水平;ELISA方法检测2组小鼠血清中Chemerin的含量;采用ELISA方法检测45例正常人及150例疑似CHD患者血清中Chemerin,其中疑似CHD患者经临床相关检测确诊为心绞痛25例,心肌梗死115例,心肌损伤非冠心病10例。结果:(1)小鼠腹主动脉旁脂肪组织中Chemerin mRNA相对表达水平HF组较NF组明显升高(P<0.01)。(2)小鼠血清中Chemerin含量HF组较NF组也明显升高(P<0.05)。(3)人血清中Chemerin含量冠心病患者(最终确诊为心绞痛及心肌梗死)较正常人群明显升高(P<0.05),其中心肌梗死患者较心绞痛患者Chemerin水平明显增加(P<0.05)。结论:在小鼠动脉粥样硬化斑块形成过程中Chemerin水平的升高提示其可能参与了促动脉粥样硬化斑块的形成。在正常人及冠心病患者尤其是心肌梗死患者的血清中Chemerin水平的改变表明其可能参与了冠心病尤其是心肌梗死的发生与发展,具有一定的预警作用。

Objective： To explore the clinical value of Chemerin in the occurrence and development of coronary heart disease（ CHD）. Methods： Healthy male Apo E- /-mice were randomly divided into normal- chow group（ NF group,n = 10） and high- fat fed group（ HF group,n = 10）. THE mRNA expression of Chemerin were measured by QPCR in mice hepatic tissue. The level of serum Chemerin were determined by ELISA. The levels of serum Chemerin in 45 cases of normal control group and in 150 cases of CHD suspected group were measured with ELISA. Results：（ 1） Chemerin mRNA relative expression levels of mice in abdominal aorta adipose tissue： in HF group was compared with in NF group,there was significantly difference（ P 0. 01）.（ 2）The serum Chemerin levels of mice： in HF group than the in NF group was significantly increased（ P 0. 05） and Chemerin level of the serum were increased（ P 0. 05）.（ 3） The levels of serum Chemerin of the normal population and patients with CHD： in CHD group（ eventually diagnosed as angina and myocardial infarction） was compared with in the normal population,there was significantly difference（ P 0. 05）,Chemerin levels of myocardial infarction was increased more than angina group（ P 0. 05）.Conclusion： Chemerin level increase in the process of formation of atherosclerotic plaques in mice may be involved in promoting atherosclerotic plaque formation. Changes of Chemerin level may be involved in the occurrence and development of CHD and MI,there is a role in early warning for CHD.