摘要
目的:考察影响高温α-淀粉酶酶解去除薏苡仁中淀粉的关键参数,为薏苡仁总多糖的应用提供参考。方法:以还原糖含量及碘液检试样品颜色变化作为定量及定性指标,在单因素试验基础上,通过正交试验考察酶解温度、酶用量、酶解时间、酶解p H对薏苡仁中淀粉酶解程度的影响。结果:最佳酶解参数为酶用量600 U·g-1,酶解温度75℃,酶解p H 6.0,酶解时间120 min;薏苡仁总多糖水提液中还原糖质量分数约25%,且碘试液检测不变色,薏苡仁总多糖纯度达76.63%,较未去除淀粉的薏苡仁总多糖纯度54.41%显著提高。结论:高温α-淀粉酶可简单、快速的去除薏苡仁中淀粉,有利于高纯度薏苡仁总多糖的获得。
Objective: To provide a reference for application of total polysaccharides in Coicis Semen by investigating effect of key parameters on high temperature α-amylase enzymolysis for starch in Coicis Semen. Method: Take the content of reducing sugar as a quantitative indicator and color change after iodine solution testing as a qualitative indicator, based on single factor tests, orthogonal test was adopted to investigate effects of the amount of enzyme, hydrolysis temperature, hydrolysis pH and reaction time on amylohydrolysis. Result: Optimal parameters were as follows: the amount of enzyme 600 U·g^-1, enzymolysis temperature 75℃, enzymatic pH of 6.0, enzymolysis time of 120 min. The content of reducing sugar in extract of total polysaccharides in Coicis Semen was about 25% , meanwhile, color of samples did not alter after iodine solution testing. Purity of total polysaceharides in Coicis Semen was 76.63% , which showed a great improvement by comparing with 54.41% of samples without removing starch. Conclusion: High-temperature a-amylase can remove starch from Coicis Semen readily and rapidly, it is conducive to extraction of total polysaccharides.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第5期16-19,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81373979)
江苏省中医药领军人才项目(LJ200913)
江苏省自然科学基金项目(BK20141058)
关键词
薏苡仁
淀粉
高温Α-淀粉酶
还原糖
酶解工艺
总多糖
Coicis Semen
starch
high-temperature a-amylase
reducing sugar
enzymolysis process
total polysaeeharides