苦豆子总碱对糖尿病胃轻瘫大鼠胃窦平滑肌作用机制的影响

Effects of Total Alkali Sophora alopecuroids on Gastro Antrum Smooth Muscle Strips in Diabetic Gastroparesis Rats

目的:观察苦豆子总碱(total alkali Sophora alopecuroids,TASa)对糖尿病胃轻瘫(diabetic gastroparesis,DGP)大鼠胃窦平滑肌活动的影响。方法:1腹腔注射链脲佐菌素建造糖尿病大鼠模型;2测定糖尿病大鼠胃内色素残留率和小肠传输速率以证实DGP模型的建立;3用恒温灌流浴槽,将标本分为正常组(NS)和DGP模型组,模型组分:TASa,TASa+异搏定(1×10-7mg·L-1),TASa+酚妥拉明(1×10-6mg·L-1),TASa+苯海拉明(1×10-6mg·L-1),TASa+消炎痛(1×10-6mg·L-1)和TASa+阿托品(1×10-6mg·L-1)6组,以探讨TASa对DGP大鼠胃窦平滑肌的作用及机制。结果:TASa能增强DGP大鼠胃窦平滑肌收缩波的振幅、持续时间和收缩面积(P<0.01),但对频率无显著影响;异搏定能明显抑制TASa收缩平滑肌的作用,而阿托品、酚妥拉明、苯海拉明和消炎痛无影响。结论:TASa可能是激活Ca2+通道而加强DGP大鼠胃窦平滑肌的收缩作用。

Objective： To study the effects of the total alkali Sophora alopecuroids （TASa） on gastro antrum smooth muscle strips in diabetic gastroparesis rats and the possible mechanisms. Method： Forty wister rats were randomly divided into the control group （10 rats） and model group （30 rats）. Rats in the model group were given intraperitoneal injection of streptozotoc to induce type I diabetic model. Weight, concentration of blood glucose, residual rate of gastric pigment and intestinal conduction velocity were detected. The isolated perfusion method was used to record amplitude, duration, area and contraction frequency of the gastro antrum smooth muscle in vitro. The strips of model group were divided into six groups： TASa group, TASa ＋ 1 ×10^-7mg·L^-1 verapamil group, TASa ＋1 ×10^-6mg·L^-1 phentolamine group, TASa ＋ 1 ×10^-6mg·L^-1 benzhydramine group, TASa ＋ 1 ×10^-6mg·L^-1 indomethacin group, TASa ＋ 1×10^-6mg·L^-1 atropine group. The contraction effects of TASa were investigated. Result： TASa could increase gastro antrum smooth muscle＇s contraction amplitude, contraction duration and contraction area, had no effect on contraction frequency. Verapamil could inhibit the gastro antrum smooth muscle contraction of TASa, but atropine, phetolamine, benzhydramine and indomethacin had no contraction effect. Conclusion： TASa may activate calcium channel, increase calcium flow to the interior of the cell and increase gastro antrum smooth muscle＇s contraction.