姜酚对家兔胸主动脉平滑肌的舒张作用及其机制

Vasodilative effect and the underlying mechanism of gingerol on isolated thoracic aorta ring of rabbits

目的 观察姜酚（gingerol）对家兔胸主动脉平滑肌的舒张作用并探讨其作用机制.方法 制备离体内皮完整的和去内皮胸主动脉环肌条,悬挂于恒温浴槽中,并将其与张力-位移换能器相连记录血管环肌条张力的变化,所有标本分为内皮完整组和去内皮组.内皮完整组分为1×10^-5 mol/L去甲肾上腺素（NE）＋不同浓度gingerol组（1×10^-8、1 ×10^-7、1 ×10^-6、1×10^-5、1×10^-4、1×10^-3 mol/L,下同）、20 mmol/L KCl＋不同浓度gingerol组、1×10^-5 mol/L NE＋左旋-硝基精氨酸甲脂（L-NAME）＋不同浓度gingerol组、1×10^-5 mol/L NE＋吲哚美辛＋不同浓度gingerol组、20 mmol/LKCl＋ L-NAME＋不同浓度gingerol组、20 mmol/L KCl＋吲哚美辛＋不同浓度gingerol组;去内皮组分为1×10^-5 mol/L NE＋不同浓度gingerol组、20 mmol/L KCl＋不同浓度gingerol组.结果 （1）gingerol能抑制1×10^-5 mol/L的NE或20 mmol/L的KCl引起的胸主动脉平滑肌收缩并呈剂量依赖性,另外,gingerol对内皮完整的胸主动脉的舒张作用较去内皮的胸主动脉明显增强（P＜0.05）.（2）内皮完整的胸主动脉组,L-NAME能显著抑制gingerol对胸主动脉平滑肌的舒张作用（％,NE处理组：94.83±3.63比96.83±1.82、91.17 ±2.89比94.83±2.68、83.83±2.23比94.67±2.73、78.00±3.54比92.33±2.91、67.83±2.37比90.83±3.77、62.83±3.42比89.67±3.15;KCl处理组：90.33±8.33比98.17±7.56、80.83±7.24比96.83±7.28、70.83±7.57比92.17±7.31、67.83±7.68比89.83±7.23、62.83±7.49比90.33±8.87、57.33±8.32比87.67±8.97,P＜0.05）.（3）内皮完整的胸主动脉组吲哚美辛能显著抑制gingerol对胸主动脉平滑肌的舒张作用（％,NE处理组：93.83±7.61比97.83±7.45、92.50±10.13比95.33±8.36、86.67±8.24比93.83±7.28、79.83±7.36比89.83±7.63、67.83±7.46比85.33±8.94、61.83 ±7.55比81.83 ±7.25;KC1处理组：90.17±7.77比96.17±7.31、79.67±8.65比91.33±8.62、72.17 ±7.48比85.67±8.76、70.83±7.78比83.17±7.79、56.83±7.62比77.83±7.29、54.17 ±7.68比74.83±7.34,P＜0.05）.结论 gingerol对NE和KCl所诱发的收缩具有剂量依赖性的抑制作用,其机制可能与内皮产生的NO和前列环素有关.

Objective To observe the effect of gingerol on the isolated thoracic aorta ring of rabbits and investigate its underlying mechanisms.Methods The thoracic aortic ring muscle strips with intact and denuded endothelium were prepared and placed in an organ chamber containing physiological salt solution （PSS）.Every strip was connected to the tension transducer and the change of the vascular ring tension was recorded and analyzed by the biological signal collection and analysis computer system.Th isotonic tension of thoracic aortic tings with intact and denuded endothelium precontracted by norepinephrine （NE,1 ×10^-5 mol/L） or KCl （20 mmol/L） was recorded before and after administrating gingerol.Meanwhile,we also observed the effects of N-nitro-L-arginine methyl ester （L-NAME,1 ×10^-4 mol/L） and indomethacin （1 ×10^-8 mol/L） on the action of gingerol in the rings.Results （1） Administration of 1 ×10^-5 mol/L NE or 20 mmol/L KCl could produce the contraction effect in the thoracic aortic rings with intact endothelium.gingerol induced the muscle relaxation of aorta in both intact and denuded endothelium groups in a dose-dependant manner,meanwhile,the relaxation effect of gingerol was stronger in intact endothelium group than that in denuded endothelium group （P 〈 0.05）.（2） L-NAME could inhibit the vasodilative role caused by gingerol in intact endothelium group （%,94.83 ±3.63 vs.96.83 ± 1.82,91.17 ± 2.89 vs.94.83 ± 2.68,83.83 ± 2.23 vs.94.67 ± 2.7,78.00 ± 3.54 vs.92.33 ± 2.91,67.83 ± 2.37 vs.90.83 ±3.77,62.83 ±3.42 vs.89.67 ±3.15 respectively in NE-treated group and 90.33 ± 8.33 vs.98.17±7.56,80.83 ±7.24 vs.96.83 ±7.28,70.83±7.57 vs.92.17±7.31,67.83 ±7.68 vs.89.83 ± 7.23,62.83 ± 7.49 vs.90.33 ± 8.87,57.33 ± 8.32 vs.87.67 ± 8.97 respectively in KCl-treated group,P 〈 0.05）.（3） Indomethacin could inhibit the vasodilative role caused by gingerol in intact endothelium group （%,93.83 ± 7.61 vs.97.83 ± 7.45,92.50 ± 10.13 vs.95.33 ± 8.36,86.67 ± 8.24 vs.93.83 ± 7.28,79.83 ± 7.36 vs.89.83 ± 7.63,67.83 ± 7.46 vs.85.33 ± 8.94,61.83 ± 7.55 vs.81.83 ±7.25 respectively in NE-treated group and 90.17 ±7.77 vs.96.17 ±7.31,79.67 ±8.65 vs.91.33 ±8.62,72.17 ±7.48 vs.85.67 ±8.76,70.83 ±7.78 vs.83.17 ±7.79,56.83 ±7.62 vs.77.83 ±7.29,54.17 ±7.68 vs.74.83 ±7.34 respectively in KCl-treated group,P〈0.05）.Conclusion （1） Gingero preferentially antagonized the contraction effect induced by NE or KCl in a dose-dependent manner.（2） The vasorelaxation effect of gingerol on the thoracic aortic ring muscle strips was relevant to the production of NO and prostacyclin.