CXC趋化因子受体5在胃癌中的表达与临床意义

CXC chemokine receptor 5 expression in gastric cancer and its clinical significance

目的 探讨CXC趋化因子受体5（CXCR5）在人胃癌细胞株及胃癌组织中的表达特点并分析其表达的临床意义.方法 采用流式细胞术检测人胃癌细胞株BGC-823、SGC-7901及HGC-27中CXCR5的表达,采用组织芯片技术及免疫组织化学方法检测90例胃癌组织及癌旁组织中CXCR5的表达,并分析其临床意义.结果 流式细胞术检测结果表明,在人胃癌细胞株BGC-823、SGC-7901及HGC-27中均能检测到CXCR5的表达;组织芯片及免疫组织化学结果显示胃癌组织及癌旁组织中均有CXCR5的表达,其在胃癌中高表达的比率为52.87％;肿瘤大小≤2.8 cm的胃癌患者其CXCR5高表达的比率显著低于肿瘤大小＞2.8 cm的胃癌患者,差异有统计学意义（x2=3.995,P＜0.05）;胃癌组织中CXCR5的表达水平与患者其他临床病理特征无明显相关（P＞0.05）.生存分析显示,CXCR5高表达的胃癌患者术后总生存率较低于CXCR5低表达的胃癌患者[风险比（HR）=1.810,95％可信区间（CI）：0.980 3～3.341 0,P＞0.05].结论 CXCR5可能与胃癌发生发展有关.

Objective To investigate the expression of CXC chemokine receptor 5 （CXCR5） in human gastric cancer cell lines and gastric carcinoma tissues and its clinical significance.Methods Flow cytometry was performed to analyze the CXCR5 expression in human gastric cancer cell lines,BGC-823,SGC-7901 and HGC-27.Immunohistochemistry and tissue-microarray were used to detect the CXCR5 expression in human gastric cancer tissues and adjacent normal tissues from 90 patients.Results The flow cytometry revealed that the CXCR5 expression was detectable in BGC-823,SGC-7901 and HGC-27 cells.The results of tissue microarray and immunohistochemistry showed that the ratio of higher CXCR5 expression in gastric cancer tissues was 52.87%.The CXCR5 expression level in patients with tumor size ≤ 2.8 cm group was significantly lower than that in patients with tumor size 〉 2.8 cm （x2 =3.995,P 〈 0.05）.There was no significant correlation between the expression of CXCR5 in gastric carcinoma and other clinical parameters （P 〉 0.05）.Survival analysis showed that the overall survival rate of the patients with high CXCR5 expression was lower than that of the patients with low CXCR5 expression [hazard ratio （HR） =1.810,95% confidence interval （CI）：0.9803-3.341 0,P〉0.05].Conclusion CXCR5 is involved in the oncogenesis and progression of human gastric cancer.