玉米秸秆分解微生物复合菌剂群落组成及提高沼气产量的研究

Community composition of biological diversity in microbial inoculum for maize straw decomposition and their effect in methane gas production

为提高农业废弃物混合厌氧发酵中沼气产率,以牛粪、秸秆等固态物料为发酵原料,采用自行设计沼气发酵装置,研究不同菌剂投量(0,100 m L,200 m L,300 m L)对沼气产量变化的影响。结果表明,与未添加菌剂的对照(T1)相比,处理组(T2、T3、T4)总产气量分别提高6.6%、19.6%、16.6%。有效证实添加微生物菌剂有助于提高沼气产量。利用变形梯度凝胶电泳(DGGE),分析复合菌剂群落,结果表明,其中的9个条带的近缘种,只有1株为未可培养菌外,其余已知菌分别归属6个属,从系统发育树可见该复合系有丰富的菌种组成多样性。

In order to rise up the gas production in process of anaerobic fermentation of agricultural waste, a setof microbial inoculum（CPB） which can degrade maize straw was build up and inoculated into the fermentation sys-tem filled with cow dung and straws to investigate the effects in gas production. Different amounts of microbial inocu-lum 0（T1）,100 mL（T2）, 200 mL（T3） and 300 mL（T4） were inoculated and the gas production was measured. The gasproduction was increased by 6.6%, 19.6% and 16.6% compared to no-CPB inoculated control, respectively. Degra-dation of cellulose-riched materials may be correlated with the lytic ability of microbial inoculum. As well as, DG-GE results shows that 6 strains were indentified and incubated except 1 strain which can not be incubated.