复方守宫散对S180荷瘤小鼠免疫功能的影响

Immunomodulatory property of Compound Shougong powder on S180 tumor bearing mice

目的 研究复方守宫散（SGS）对S180荷瘤小鼠免疫功能的影响.方法 将S180细胞株分别接种于昆明种小鼠制备荷瘤小鼠模型,待肿瘤模型制备成功后随机分为模型对照组、阳性对照组及SGS（9.75、19.50、29.25 g/kg）组,另设健康小鼠为正常对照组,除正常对照组外其余各组连续灌胃给药7d,每天1次.各组分别采用碳廓清试验、溶血素生成试验、DNFB诱导的迟发性超敏反应,观察SGS对荷瘤小鼠非特异性免疫、体液免疫和细胞免疫功能的影响.结果 与模型对照组相比,SGS组荷瘤小鼠的胸腺和脾脏脏器指数均提高（均P＜0.05）,且碳廓清指数和吞噬指数提高（均P＜0.05）,血清IgM、IgG的生成和脾细胞溶血素的产生增多（均P＜0.05）,CD4^＋、CD8^＋细胞数和CD4^＋/CD8^＋的比值及脾淋巴细胞IL-2的含量升高（均P＜0.05）.结论SGS通过刺激荷瘤小鼠的特异性和非特异性免疫应答,对荷瘤小鼠的免疫功能有良好的增强作用.

Objective To study the immunomodulatory property of Compound Shougong powder（SGS） on S180 tumor-bearing mice.Methods The S180 model of tumor-bearing mice by Kunming mice were prepared.Then,the tumor-bearing mice were randomized into the model group,positive control group,and SGS groups （29.25g/kg,19.50g/kg,9.75g/kg）.Besides the normal group,the other groups were used intragastric administration of into tumor-bearing mice by one time,14 consecutive days.The carbon clearance,quantitative hemolysis and DNFB induced delayed-type hypersensitivity were applied to assay effects of SGS on nonspecific immunity,humoral immunity and cellular immunity.Results Compared with the model control group,in the SGS groups,the tumor-bearing mice with spleen and thymus index of organ improved （all P 〈 0.05）,and the clearance index and values of phagocytic index were elevated（all P 〈0.05）,and the productions of IgM and IgG in serum and hemolysin in splenocytes were enhanced（all P 〈0.05）,and the percentages of T cells expressing CD4^＋,CD8^＋ and the ratio of two subset of T lymphocyte increased,and the IL-2 production of spleen lymphocytes was also improved （all P 〈 0.05）.Conclusion SGS showed significant immunomodulatory property on tumor-bearing mice through specific and nonspecific immunity.