蚕蛹水解氨基酸对人肝癌细胞HepG2.2.15的体外抑制作用的研究

Inhibition effect of hydrolytic amino acid of silkworm chrysalis( HAASC) to the Hep G2.2.15

目的探讨蚕蛹水解氨基酸对Hep G2.2.15(人肝癌细胞株)在体外的抑制作用。方法运用MTT比色法测定蚕蛹水解氨基酸对人QSG-7701(正常肝脏细胞株)的毒性后,计算蚕蛹水解氨基酸的安全浓度。将蚕蛹水解氨基酸溶液分别配制成0.001 mg/ml、0.01 mg/ml、0.1 mg/ml、1 mg/ml和10 mg/ml的浓度,与Hep G2.2.15细胞共同培养,实验同时设置空白对照和细胞对照组。每组设置4个复孔,每孔加入100μl的5×104个/ml的细胞悬液,于培养的24、48、72小时后,用MTT比色法测定OD值,评定蚕蛹水解氨基酸对Hep G2.2.15细胞株的抑制作用。结果蚕蛹水解氨基酸的最大无毒浓度(TC0)为12 mg/ml。梯度浓度的蚕蛹水解氨基酸与Hep G2.2.15细胞共同培养24、48和72 h后,与细胞对照组相比,OD值的差异有显著性(P<0.05),并有时间和浓度的线性关系。结论蚕蛹水解氨基酸对人肝癌细胞Hep G2.2.15具有显著的抑制作用,且毒性较低。

Objective To discusses the inhibition effect of hydrolytic amino acid of silkworm chrysalis （HAASC） to the HepG2.2.15.Methods The safe concentration of HAASC were obtained by MTT way to detect the cell toxicity of the HAASC to QSG-7701.And then, different concentration （0.001 mg/ml, 0.01 mg/ml, 0.1 mg/ml, 1 mg/ml and 10 mg/ml） of HAASC were added to every hole respectively , and blank control group ,cell control group were sent in the meantime .Each group was set up four holes .Each group was joined 100μl cell suspension with 5 ＆#215;104 /ml.which to raise 24, 48 and 72h.At last, estimating the restraining effect of HAASC to HepG2.2.15 by MTT ways to detecting OD value .Results The TC0 of HAASC was 12mg/ml.The OD value of different concentration of HAASC were different after 24,48 and 72h, later, comparing to cell control, and has significance （P〈0.05）, which had cable sex with time and concentration .Conclusions Hydrolytic amino acid of silkworm chrysalis has a significant inhibitory effect on the human hepatic cancer cells HepG2.2.15 and has low toxicity .