摘要
目的建立了二维液相色谱法测定保健食品中VA、VD3、VE含量的方法。方法采用甲醇与乙醇超声直接提取样品,以Eclipse Plus C18(2.1 mm×100 mm,1.8μm)为一维色谱柱,流速为0.5 ml/min;以poroshell 120(4.6 mm×100 mm,2.7μm)为二维色谱柱,流速为1.0 ml/min;采用波长切换方式检测(VA:325 nm;VD3:264 nm;VE:296 nm),以乙腈-甲醇-水为流动相,梯度洗脱,柱温为35℃。结果维生素A在0.21μg/ml^8.38μg/ml、维生素D3在0.04μg/ml^1.73μg/ml、维生素E在2.05μg/ml^81.84μg/ml线性关系良好,相关系数r均为1.000 0,平均回收率为95.6%~102.7%。结论本方法快速、准确、重现性好,适用于保健食品复合维生素片或软胶囊中VA、VD3、VE的测定。
Objective To develop a two- dimensional liquid chromatography method for the content determination of VA,VD3,VEin functional food. Methods Samples were directly extracted by methanol and ethanol with the assitance of ultrasonic,Eclipse Plus C18 as first dimensional column（ 2. 1 mm × 100 mm,1. 8 μm）,with flow rate at 0. 5 ml / min; Proshell 120 as second dimensional column（ 4. 6 mm × 100 mm,2. 7 μm）,with flow rate at 1. 0 ml / min; Detection was conducted when wavelength were 325 nm,264 nm and 296 nm respectively; Methanol,acetonitrile and water as the mobile phase,gradient elution,the column temperature was 35 ℃. Results The linear range of VA,VD3,VEwas good among 0. 21 μg / ml- 8. 38 μg / ml,0. 04 μg / ml- 1. 73 μg / ml,and 2. 05 μg / ml- 81. 84 μg / ml,respectively,r = 1. 000 0,the average recovery was 95. 6%-102. 7%. Conclusion The established method was fast,accurate and with good reproducibility for the content determination of VA,VD3,VEin soft capsules and multivitamin tablets.
出处
《中国卫生检验杂志》
CAS
2015年第3期340-343,347,共5页
Chinese Journal of Health Laboratory Technology
