摘要
目的探讨姜黄素对6-羟基多巴胺(6-OHDA)诱导的PD大鼠模型的精神保护作用及作用机制。方法应用6-OHDA分别建立帕金森病(PD)动物模型和细胞模型。从行为学角度(旋转实验),观察姜黄素预处理对6-OHDA制备的PD模型大鼠的保护作用并通过观察各组细胞线粒体跨膜电位、细胞活性氧物质(ROS)以及检测细胞中Caspase-3的含量变化初步探讨其作用机制。结果 1动物模型:PD模型组大鼠旋转圈数明显增加(平均316 r/30 min),与空白组(0 r/30 min)相比,差异有统计学意义(P<0.05)。应用姜黄素预处理后,大鼠旋转圈数明显减少(206 r/30 min),与PD模型组相比,差异有统计学意义(P<0.05)。2细胞模型:姜黄素可以通过抗氧化应激的作用对多巴胺能神经细胞阻断由6-OHDA所导致的细胞线粒体跨膜电位(Δψm)的下降,抑制细胞内ROS水平;也可以通过抗凋亡的作用减轻Caspase-3的活化。结论姜黄素对6-OHDA制备的PD大鼠模型具有保护作用,可能是通过抗氧化应激、抗凋亡等机制来发挥保护作用。
Objective To study the neuroprotective effect and mechanism of curcumin on 6-OHDA-induced Parkinson＇s disease（PD） rat model. Methods Rat model and cell model of PD were established by 6-OHDA. From the behavioral point of view（rotation experiment）, the protective effect of curcumin pretreated 6-OHDA PD rat model was observed and the mechanism of curcumin was explored through observing the cell mitochondrial membrane potential, cellular reactive oxygen species and the content change of Caspase-3 cells in each group of cells. Results ① Rat model： PD rat model group significantly increased the number of slewing ring（an average of 316 r/30 min）, compared with the blank control group（0 r/30 min）, and the difference was significant（P〈 0.05）. Rats with curcumin pretreatment had significantly decreased slewing rings（206 r/30 min）, and compared with the PD model group, the difference was significant（P〈 0.05）. ② Cell model： Curcumin on 6-OHDA processing MES 23.5 dopaminergic cells blocked the Δψm drop caused by 6-OHDA, which can resist to oxidative stress, inhibit intracellular ROS levels and reduced the activation of the Caspase-3 by antiapoptotic effect. Conclusion Curcumin plays a protective role in 6-OHDA-induced PD rat model. Its mechanism may be the resistance to oxidative stress and antiapoptotic mechanisms.
出处
《中南药学》
CAS
2015年第1期34-37,共4页
Central South Pharmacy
