黄芩提取物对常见致病菌的体内外抑菌活性测定

Extract Effects of Scutellaria baicalensis on the Antibacterial Activity of Common Pathogenic Bacteria in Vitro and in Vivo

为观察黄芩提取物对金黄色葡萄球菌、大肠杆菌和沙门氏菌等致病菌的抑菌活性,试验采用试管二倍稀释法,测定黄芩提取物对金黄色葡萄球菌、大肠杆菌和沙门氏菌的体外抑菌活性;采用相同浓度致病菌灌胃小鼠后,分别用黄芩提取物灌胃治疗,测定小鼠十二指肠、空肠、回肠和盲肠中肠道菌群数量。结果表明,无论在体外还是体内,黄芩提取物对金黄色葡萄球菌、大肠杆菌和沙门氏菌均具有抑制作用,且对金黄色葡萄球菌抑制性最强,对大肠杆菌和沙门氏菌抑制性较弱;且通过灌注黄芩提取物,显著降低了各致病菌致病后小鼠的死亡率。通过研究得出,黄芩提取物对常见的金黄色葡萄球菌、大肠杆菌和沙门氏菌等致病菌具有较明显的体内外抑菌作用。

To observe the antibacterial activity of scutellaria baicalensis extract against staphylococcus aureus,escherichia coli and salmonella,the antibacterial activity of scutellaria baicalensis extract against staphylococcus aureus,escherichiacoli and salmonella were determined in vitro by the test of tube two times dilution method. Scutellaria baicalensis was injected into stomach of the treated mice by the same concentration of staphylococcus aureus,escherichiacoli and salmonella in vivo,the number of bacteria in duodenum,jejunum,ileum and cecum of mice was determined respectively. The results showed that scutellaria baicalensis extract had the inhibitory effects on staphylococcus aureus,escherichia coli and salmonella,and suppress the strongest inhibition on staphylococcus aureus,the weaker of escherichia coli and salmonella,and the perfusion of scutellaria baicalensis extract significantly reduced the mice mortality of each pathogen causing. The research showed that the antibacterial effects of scutellaria baicalensis extract on staphylococcus aureus,escherichia coli and salmonella was obvious in vivo and in vitro.