以线虫为模型研究反式脂肪酸的毒理作用

Toxicological Action of Trans Fatty Acids by Using Caenorhabditis elegans as Model

首次以秀丽新杆线虫为模型,研究了反式脂肪酸(TFA)对于连续3代线虫寿命及生育能力的影响,从而考察反式脂肪酸可能的毒理作用。结果发现:在寿命试验中,5%TFA能够显著缩短亲代、F1代、F2代线虫的寿命;0.05%、0.5%TFA能够不同程度地缩短F1代、F2代线虫的寿命,并且具有多代累积作用。在产卵试验中,0.05%TFA对亲代、F1代线虫的生殖能力没有明显影响,但是0.5%、5%TFA能够不同程度地降低亲代、F1代线虫的生殖能力。而在F2代中,0.05%、0.5%、5%TFA均能显著降低线虫的生殖能力,说明0.05%、0.5%、5%TFA对线虫生殖能力的负面影响同样具有多代累积的作用。

To investigate toxicological effects of TFA,using C. elegans worms as model,we studied the effects of trans fatty acids on lifespan and fertility of C. elegans worms of three running generations. The results of the lifespan experiment showed that 5% TFA could significantly shorten the lifespan of parental worms,F1 worms and F2worms; 0. 05% and 0. 5% TFA could to varying degrees shorten the lifespan of F1 worms and F2 worms,and had the effect of accumulation over multi-generations. The results of the oviposition experiment showed that 0. 05% TFA had no significant effect on the fertility of parental worms and F1 worms,but 0. 5% and 5% TFA could to varying degrees reduce the fertility of parental worms and F1 worms. In F2 worms,0. 05%,0. 5% and 5% TFA could significantly reduce the fertility of C. elegans,indicating that the negative effects of 0. 05%,0. 5% and5% TFA on fertility were characteristic of accumulation over multi-generations.