消岩汤对耐顺铂人肺腺癌A549/DDP细胞多药耐药基因调控作用的研究

Regulation function of Xiaoyan Decoction on multidrug resistance of lung adenocarcinoma A549/DDP cell with cisplatin resistance in human gene

目的探讨具有"扶正解毒祛瘀"作用的中药复方消岩汤对耐顺铂人肺腺癌A549/DDP细胞多药耐药基因(MDR)的表达产物P糖蛋白(P-g P)及其m RNA表达水平的影响。方法选择耐顺铂人肺腺癌A549/DDP细胞系作为获得性耐药模型,给予消岩汤含药血清,采用Western Blotting免疫印迹法及RT-PCR技术检测MDR的表达产物P-g P的含量及其m RNA表达水平。结果与对照组比较,随着消岩汤含药血清药物浓度的增加,P-g P蛋白表达逐渐减弱(P<0.05);与对照组比较,经不同浓度消岩汤含药血清作用后,A549/DDP细胞中的MDR1基因的m RNA水平均有所下降(P<0.05),且随着药物浓度的增加,基因表达抑制作用越明显。结论消岩汤逆转肺癌耐药的可能机制为通过抑制细胞膜上P-g P及其基因的表达而阻止细胞对顺铂的输出,从而聚集细胞内的药物浓度达到有效逆转肺癌耐药的效果。

Objective To study the Chinese materia medica compound （Xiaoyan Decoction） against lung adenocarcinoma A549/DDP cell multidrug resistance （MDR） in human gene expression product of P glycoprotein （P-gP） and its mRNA expression level influence, to find Xiaoyan Decoction target resistant to chemotherapy, to overcome the drug resistance in lung cancer research foundation laid. Methods The choice of model as acquired resistance to cisplatin resistance of human lung adenocarcinoma A549/DDP cell line, the application of serum pharmacology method, using Westem blotting and RT-PCR technique for the detection of Xiaoyan Decoction on MDR of A549/DDP cell gene expression product of P-gP and its effect on mRNA expression level. Results Compared with the normal saline group, with the serum drug concentration of Xiaoyan Decoction increased and the expression of P-gP protein decreased gradually （P 〈 0.05）; Compared with the saline group, serum was treated with different concentration of Xiaoyan Decoction after the action of MDR1 gene in A549/DDP cells and the mRNA levels were decreased, and with the increase of drug the concentration, the more obvious inhibition of gene expression was observed （P 〈 0.05） Conclusion The possible mechanism of Xiaoyan Decoction reversal of drug resistance in lung cancer is obtained by inhibiting the expression of P-gP on the cell membrane and its gene and the cell output to cisplatin could be prevented, thus the aggregation of the intracellular drug concentration effectively could reverse the effect of drug resistance in lung cancer.