右归丸干预自身免疫性卵巢早衰小鼠EGF的实验研究

Experimental research on intervening EGF with Yougui Wan in mice with autoimmune premature ovarian failure

目的:观察右归丸对自身免疫性卵巢早衰小鼠模型血清FSH、E2、EGF及卵巢形态等的影响,以探讨右归丸的作用机理。方法:建立透明带抗原自身免疫性POF模型小鼠,并将其随机分为五组,空白组、模型组正常饮食、中药组(右归丸组)、西药组(补佳乐+黄体酮组)、中西组(右归丸+补佳乐+黄体酮组)。采用酶联免疫法检测血清中表皮细胞生长因子(Epidermal growth factor,EGF)水平,光镜下观察卵巢组织学形态,应用SPSS22.0进行统计分析。结果:造模后小鼠动情周期延长;光镜下观察卵巢组织,较空白组生长卵泡、黄体数少,闭锁卵泡多;血清中FSH、EGF值高于空白组(P<0.05),E2值低于空白组(P<0.05)。右归丸治疗后动情周期缩短;光镜下小鼠卵巢组织生长卵泡、黄体数较模型组增多,闭锁卵泡减少;血清FSH、EGF值降低(P<0.05),E2值升高(P<0.05)。中药组、西药组和中西组在对性激素及EGF水平的影响上无明显差异(P>0.05)。结论:1通过组织形态学、细胞生物学等研究证实右归丸能够从多方面促进小鼠卵泡生长、发育,改善卵巢功能;2右归丸能降低POF小鼠血清EGF水平;3EGF可有效反映自身免疫性POF模型小鼠卵巢功能的变化,可作为临床检测卵巢功能的指标。

Objective： To observe clinical effects of Yougui Wan on intervening serum FSH, E2, EGF and ovarian morphology in autoimmune premature ovarian failure mice model. Methods：POF model with autoimmune micezona pellucida antigen were randomly divided into five groups, as blank group, model group, TCM group （Yougui Wan group）, western medicine group （Progynova＋progesterone group）, the integrative medicine group （Yougui Wan＋Progynova＋progesterone group）. EGF levels in epidermal growth factor serum were observed by light microscope ovarian histology morphology. Results：After modeling, estrous cycle in mice was prolonged;ovarian tissue was observed by light microscope, growing follicles and corpus luteum was fewer than that in the blank, but the follicleatresia was in the contrary;FSH, EGF levels in serum was higher than that in the blank （P0.05）. Conclusion： I. Depending on organizations morphology and cell biology research, Yougui Wan can promote the mice follicle growth, development and improve ovarian function;II. Yougui Wan can reduce EGF level in POF mice serum;III. EGF can effectively reflect the changes of ovarian function in autoimmune POF mice model, can be used as indicators in ovarian function detection.