摘要
利用α-糖基转移酶催化玉米淀粉制备了纯度约为46％的大环糊精.同时利用Bio-Gel P-4（Fine,45～ 90 μm）柱层析对大环糊精进行初步分离,通过硅胶（200～ 300目）柱层析进一步分离,用薄层色谱法和高效液相法对分离液进行鉴别和纯度分析,分别得到纯度为92.4％、90.2％、86.7％、84.5％、85.3％的11-15单一聚合度大环糊精.
Cycloamylose with the purity about 46% is prepared by catalyzing the corn starch using a- glyeosyhransferase. Preliminary separation of obtained eycloamylose is performed by Bio-Gel P-4 （Fine,45-90） column chromatography. Silica gel is used to further purify the cycloamylose. TLC and HPLC are used to identify the separation process and analyze the purity of the resulting cycloamylose. It shows that the purity of cycloamylose with single degree of polymerization between II and 15 is 92.4% ,90. 2% ,86. 7% ,84. 5% and 85.3% ,respectively.
出处
《现代化工》
CAS
CSCD
北大核心
2014年第12期54-57,共4页
Modern Chemical Industry
基金
江西省教育厅资助项目(GJJ11290)
关键词
大环糊精
分离
柱层析
cycloamylose
separation
column chromatogrphy
