病理组织厚切片抗酸染色在结核病诊断中的价值

Value of pathological diagnosis in tuberculosis by biopsy and thick section acid-fast staining

目的探讨病理活检组织厚切片抗酸染色在结核病诊断中的价值。方法通过66例组织学HE光镜检查见典型或不典型结核形态学改变的病理活检组织,同时进行6μm厚切片及4μm常规切片进行抗酸染色,对其结果进行比较分析。结果 6μm厚切片抗酸染色总的阳性率为48%,典型的结核病(TB)总的阳性率为58%,不典型TB阳性率为35%。而4μm常规切片总的阳性率为30%,典型TB阳性率为40%,不典型TB阳性率为15%,厚切片及常规切片阳性率比较差异具有统计学意义。结论在临床及病理活体组织常规检查的基础上,辅助组织厚切片抗酸染色,可为结核病的诊断提供更确切的诊断依据。

【Objective】To explore the value of biopsy with thick section using acid-fast staining in pathological diagnosis of tuberculosis（TB）.【Methods】A total of 66 cases of biopsy tissues which were clinically diagnosed as TB or had typical or non-typical pathological changes of TB in HE staining were divided into 6μm and regular 4μm section groups using acid-fast staining and then analyzed. 【Results】In thick 6μm section group, the positive rate was 48% for all, 58% for typical TB and 35% for non-typical TB. In regular 4μm section group, the positive rate was 30% for all, 40% for typical TB and 15% for non-typical TB. There was a significant difference between the thick section group and regular section group.【Conclusions】Thick section with acid-fast stain can help to provide more accurate evidence for the diagnosis of TB, based on clinical data and regular pathological biopsy.