NGF电纺纤维的体外缓释研究

In Vitro Sustained Release Research of NGF Electrospun Fibers

目的:研究担载神经生长因子(NGF)的静电纺丝纤维的表征,考察NGF电纺纤维对于周围神经修复的效果。方法:将NGF水溶液分散于PLLA溶液,通过W/O乳液法制备静电纺丝纤维,对纤维的形态、力学性能等进行表征,Elisa方法测定NGF的体外释放动力学,Alamer Blue法检测试剂来考察纤维释放液对于PC12细胞增殖的影响。结果:NGF电纺纤维具备良好的形态和力学性质,直径为500-900 nm,纤维具备三维多孔结构。纤维的最大拉伸应力为2.50±0.41 MPa。电纺纤维中NGF在体外可有效释放9天,累积释放量接近3000 pg。细胞活性实验结果显示,第1、3、5、7天释放液的荧光强度与对照组相比有显著差异。结论:担载NGF的乳液法静电纺丝纤维有促进缺损周围神经修复的潜质。

Objective： To investigate characterization and effect on peripheral nerve repair of NGF electrospun fibers. Methods：NGF solution was dispersed into PLLA solution, and then electrospun fibers were fabricated by W/O emulsion method. The morphology and mechanical properties of the electrospun fibers were characterized and release kinetic of NGF in fibers was detected by NGF Elisa kit. The effect of release solution on cell activity was tested by Alamer Blue. Results： The NGF emulsion electrospun fibers had good morphology and mechanical properties. The fibers had porous structure with diameters between 500 nm and 900 nm. The maximum tensile stress of fibers was 2.50±0.41 MPa. NGF in fibers had sustained release for 9 days in vitro with a cumulative release of nearly3000 pg. Statistical difference was found in fluorescence intensity of release solution compared to blank group in cell viability experiment. Conclusions： NGF emulsion electrospun fibers provide a promising approach in the application of peripheral nerve repair.