MMP-2、Galectin-3、Cx43与胃癌侵袭和转移的关系

The Relationship between Invasion and Metastasis of Gastric Cancer and MMP-2, Galectin-3 and Cx43

目的:通过检测胃癌组织和癌旁组织中MMP-2、Galectin-3、Cx43的表达情况,研究它们与胃癌发生、发展、侵袭及转移的关系。方法:应用免疫组织化学(SP法)法检测MMP-2、Galectin-3、Cx43在胃癌组织和癌旁组织中的表达情况。结果:MMP-2、Galectin-3在胃癌组织中的阳性表达均明显高于胃癌癌旁组织(P<0.05),并且均与浸润深度、淋巴结转移情况和临床分期明显相关(P<0.05);MMP-2与胃癌的分化程度有关,而Galectin-3与胃癌的分化程度无关;Cx43在20例胃癌癌旁组织中的阳性表达率达100%,在胃癌组织中阳性表达率为39.7%,在癌旁组织中的阳性表达明显高于胃癌组织,具有统计学意义(P<0.05),Cx43的表达在胃癌的分化程度、浸润深度、淋巴结转移情况及临床分期方面存在显著差异,具有统计学意义(P<0.05);MMP-2阳性表达与Cx43阳性表达呈负相关(P=0.02,r=-0.292),而与Galectin-3阳性表达均为正相关(P=0.003,r=0.344)。结论:在胃癌的发生、发展过程中Galectin-3与MMP-2有促进作用,而Cx43有抑制作用,三者在胃癌的侵袭和转移中均发挥重要作用。

Objective： To study the relationship between development, invasion and metastasis of gastric cancer and MMP-2,Galectin-3 and Cx43 by detecting the expression of Cx43 MMP-2 and Galectin-3 in gastric cancer tissues and adjacent tissues. Methods：Immunohistochemistry（SP method） was used to detect the expression of MMP-2, Galectin-3, Cx43 in gastric cancer tissues and adjacent tissues. Results： The positive expression level of MMP-2, Galectin-3 in gastric carcinoma were significantly higher than the adjacent gastric cancer tissues（P〈0.05）, and were significantly associated with depth of invasion, lymph node metastasis and clinical stage（P〈0.05）; The positive expression level of MMP-2 had related to the degree of differentiation of gastric cancer, but Galectin-3 had nothing to do with the degree of differentiation of gastric cancer. In 20 cases, the positive expression rate of Cx43 was 100% in adjacent gastric cancer tissues, and 39.7% in gastric cancer tissues. So the positive expression rate of Cx43 in adjacent gastric cancer tissues was statistically significantly higher than in gastric cancer, depth of invasion, lymph node metastasis and clinical staging had different effect on the positive expression of Cx43（P〈0.05）; The positive expression level of MMP-2 and Cx43 were negatively correlated（P= 0.02, r=-0.292）, and the positive expression level of MMP-2 and Galectin-3 were positively correlated（P = 0.003, r = 0.344）. Conclusion：Galectin-3 and MMP-2 promote the development of gastric cancer, Cx43 inhibit it. All the three play an important role in the invasion and metastasis of gastric cancer.