DAPK1、hTERT在口腔鳞癌中的表达及其临床意义研究

Expressions and Clinical Significances of DAPK1, hTERT in the Oral Squamous Cell Carcinoma

目的:探讨凋亡相关蛋白激酶1(DAPK1)、端粒酶催化亚单位(hTERT)在口腔鳞状细胞癌(OSCC)中的表达,并探讨其临床意义。方法:应用免疫组织化学方法检测DAPK1、hTERT在93例OSCC组织及10例癌旁正常组织中的表达,并分析其与OSCC临床病理参数之间的关系及其在OSCC浸润前沿中的作用。结果:癌旁正常组织中DAPK1的表达显著高于OSCC组织中DAPK1的表达,且高、中、低分化OSCC组织中DAPK1的表达比较均有显著差异(P<0.05),OSCC浸润前沿组织中DAPK1的表达低于非前沿部分(P<0.05),而DAPK1的表达与OSCC浸润前沿的IFG总分无显著相关性(P>0.05)。癌旁正常组织中hTERT的表达显著低于OSCC组织中hTERT的表达(P<0.05),且高、中、低分化OSCC组织中hTERT的表达比较均有显著差异(P<0.05);OSCC浸润前沿组织中hTERT的表达高于非前沿部分(P<0.05),且与OSCC浸润前沿的IFG总分有关(P<0.05)。DAPK1、hTERT的表达均与OSCC患者的性别、年龄、淋巴结转移无显著相关性(P>0.05)。结论:口腔鳞状细胞癌组织,特别是其前沿组织中DAPK1的表达显著下调和hTERT的表达明显上调,可能通过阻碍口腔鳞状细胞癌细胞凋亡,共同促进口腔鳞状细胞癌的生长、分化和浸润。

Objective： To investigate the Death-related protein kinase 1（DAPK1） and human telomerase revere transcriptase（hTERT） expressions in oral squamous cell carcinoma（OSCC） and explore their clinical significances. Methods： The expression of DAPK1 and hTERT in 93 cases of OSCC tissues and 10 cases of adjacent normal tissues were detected by immunohistochemistry and their correlations with the clinicopathological parameters of OSCC and OSCC infiltration front were analyzed. Results： The expression of DAPK1 in normal oral mucosa was significantly higher than that of the OSCC tissue（p〈0.05） and significant differentces were observed between those of well-differentiated, moderately differentiated and poorly differentiated OSCC tissues（P〈0.05）. The expression of DAPK1 in infiltration front was obvioulsly lower in the front part of OSCC than that in the non- front part of OSCC（P〈0.05）, but no significant correlation was found in the DAPK1 expression with IFG scoress in ITF（P〉0.05）. The expression of hTERT in normal tissue adjacent to carcinoma was significantly lower than that of OSCC tissue（P〈0.05） and significant differentces were found between well-differentiated, moderately differentiated and poorly differentiated OSCC tissues（P〈0.05）. The expression of hTERT in infiltration front was significantky higher than that of the front part of OSCC（P〈0.05） and was associated with the IFG scores of infiltration of OSCC（P〈0.05）.Both the expression of DAPK1 and hTET had no significant correlation with the sex, age and lymph node metastasis of OSCC patients（P〉0.05）. Conclusion： DAPK1 expression was significantly down-regulated in OSCC tissue and hTERT expression was significantly up-regulated in OSCC tissue, especially in the frontier of OSCC, which could promote the growth, differentiation and infiltration of OSCC through inhibiting the apoptosis of OSCC cells.