白藜芦醇对人骨髓基质细胞增殖及成骨分化的影响被引量：1

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摘要目的观察白藜芦醇对体外培养人骨髓基质细胞(BMSCs)增殖及成骨分化的影响。方法用传代贴壁筛选的方法分离纯化人BMSCs,加入白藜芦醇后1、2、3 d用MTT法检测白藜芦醇对BMSCs增殖的作用,加白藜芦醇后3、5、7、9 d用PNPP法观察白藜芦醇对BMSCs成骨诱导后细胞碱性磷酸酶(ALP)活性的影响。结果传代贴壁筛选的方法可得到BMSCs,经诱导后可向成骨细胞方向分化。10-4mol/L浓度的白藜芦醇可以抑制人BMSCs的增殖。白藜芦醇可以增加人BMSCs成骨诱导后细胞ALP的含量,其中以10-6mol/L浓度作用7 d时效果明显。结论白藜芦醇能促进人BMSCs向成骨细胞方向分化。

作者蔡德许卫铭陈航李雄

机构地区广东医学院附属医院药学部广东医学院药理学教研室广东医学院附属医院骨科广东省珠海市人民医院药学部

出处《广东医学》 CAS CSCD 北大核心 2014年第20期3140-3142,共3页 Guangdong Medical Journal

基金 2012年广东省第一批产业技术研究与开发资金计划项目(编号:2012B031800225) 2011年度广东省湛江市第二批非资助科技攻关计划项目(编号:2011C3102022)

关键词白藜芦醇骨髓基质细胞成骨细胞增殖分化

分类号 R285.5 [医药卫生—中药学]

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