切口痛大鼠皮下注射瑞芬太尼对痛觉敏感性和脊髓背角NRF-1与NR2B表达的影响

The expression of NRF-1 and NR2B in spinal dorsal horn in a rat model of remifentanil induced post-incisional hyperalgesia

目的观察切口痛大鼠皮下注射瑞芬太尼后对痛觉敏感性和脊髓背角NRF-1与NR2B表达的影响,探讨NRF-1-NR2B通路在瑞芬太尼诱发术后痛觉过敏中的作用。方法 SD雄性大鼠80只随机分为对照组（C组）：生理盐水;手术组（S组）：切口痛＋生理盐水;瑞芬太尼组（R组）：瑞芬太尼;手术＋瑞芬太尼组（SR组）：切口痛＋瑞芬太尼。瑞芬太尼皮下输注速率2.66μg·kg-1·min-1。于术前24h及术后2、6、12、24和48h测定机械缩足阈值（PMWT）和热缩足潜伏期（PWTL）。应用免疫荧光染色检测术侧L4~6脊髓节段背角NRF-1与NR2B的水平。结果与术前24h比较,术后不同时点S组和SR组PMWT明显降低、PWTL明显缩短（P〈0.05）。与C组比较,术后不同时点S组和SR组PMWT明显降低、PWTL明显缩短（P〈0.05）。与S组和R组比较,术后不同时点SR组PMWT明显降低、PWTL明显缩短（P〈0.05）。与C组比较,术后48hS组、SR组术侧脊髓背角NRF-1和NR2B的MOD明显升高（P〈0.05）。与S组和R组比较,术后48hSR组术侧脊髓背角NRF-1和NR2B的MOD明显升高（P〈0.05）。结论皮下注射瑞芬太尼提高足跖切口痛大鼠的术后痛觉敏感性,并增加术侧脊髓背角NRF-1与NR2B的表达,提示脊髓背角NRF-1和NR2B可能参与瑞芬太尼诱发术后痛觉过敏中的形成。

Objective To observe the pain sensitivity together with expression of NRF-1and NR2 Bin dorsal horn in the rat model of incisional pain after subcatenous administration of remifentanil.Methods Eighty male SD rats were randomized into four groups：group C,Saline;group S：saline and surgical incision;group R：remifentanil;group SR：remifentanil and surgical incision.The infusion rate of subcatenous remifentanil was 2.66μg·kg-1·min-1.The PWMT and PWTL were measured at different time points of 24 hbefore surgery and 2,6,12,24 and 48hafter surgery in all rats.The immunofluorescence detected the expression of NRF-1and NR2 Bin dorsal horn of L4-6.Results Compared with group C,The PWMT and PWTL were significantly lower in group S and group SR（P〈0.05）,and group SR was significantly lower than group S and R（P〈0.05）.Compared with group C,the expression of NRF-1and NR2 Bwere significantly higher in group S and group SR（P〈0.05）,and group SR was significantly higher than group S and R（P〈0.05）.There were no significant differences in the expression of NRF-1and NR2 Band the value of PWMT and PWTL between group C and R.Conclusion Subcatenous administration of remifentanil increased the pain sensitivity of rats after plantar incision,as well as the expression of NRF-1and NR2 Bin spinal dorsal horn.This suggests that NRF-1and NR2 Bin spinal dorsal horn may be involved in the formation process of postoperative hyperalgesia indouced by remifentanil.