摘要
研究病毒感染对白斑综合症病毒基因的录调控的影响,将Caspase调控区的DNA进行生物素标记,然后与链霉亲和素修饰的琼脂糖结合.通过下拉发现,Caspase基因的启动子序列与white spot syndromic virus(WSSV)病毒的两个蛋白Vp38和Vp41B有相互作用.通过荧光素酶报告基因发现,Vp38和Vp41B对Caspase启动子活性分别有抑制和激活作用.采用RNAi技术下调两个WSSV蛋白的表达后,研究发现:Vp38和Vp41B分别对对虾血细胞的凋亡水平有促进和抑制的调控作用.
The prawn Caspase gene was previously found to play important roles in protecting host from viral infection.In order to further delineate the transcriptional regulation of Caspasein response to viral infection,the Caspase promoter was biotin-labeled and bind to streptavidin-beads.Through pulldown assay the Caspase promoter was founded to interact with two white spot syndromic virus（WSSV）proteins Vp38 and Vp41B.Luciferase reporter assay revealed that Vp38 and Vp41 Bact as repressor and activator of Caspasetranscription respectively.RNAi inhibition of Vp38 and Vp41 Bindicate the two WSSV proteins play roles on the regulation of prawn hymocyte apoptosis.These results provided insights into the immune mechanism of invertebrates,and will also be helpful for shrimp viral disease control.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2014年第5期537-541,共5页
Journal of Huaqiao University(Natural Science)
基金
国家自然科学基金资助项目(31272684)
国家海洋局海洋生物遗传资源重点实验室开放研究基金项目(HY201102)
