希腊海岸松叶中聚戊烯醇含量与化学结构分析

Determination of the Content and Structure of Polyprenols from Greek Pinus pinaster Leaves

利用HPLC与聚戊烯醇对照品保留时间对照的定性方法,确定海岸松叶中聚戊烯醇的异戊烯基单元数为14~20;利用^1H NMR和^13C NMR确定海岸松聚戊烯醇化学结构为ω-trans2-cisn-OH构型。采用Kromasil C18 ODS-1（150 mm×4.6 mm,5μm）色谱柱,以甲醇-异丙醇（体积比9∶16）为流动相,流速为0.50 mL/min,检测波长为210 nm,柱温为25℃,PDA检测器等色谱条件,测定海岸松叶聚戊烯醇在1.10~12.10μg范围内线性良好（r=0.996 3）,平均回收率为98.21%,RSD为2.27%（n=6）,海岸松干叶中聚戊烯醇含量为0.33%。

The isopentenyl unit numbers of polyprenols from Greek Pinus pinaster leaves was 14 to 20 by contrasting the retention time of polyprenol reference substance using HPLC. And the polyprenols structure was confirmed as ω-trans2-cisn-OH by^1 H and ^13C spectrum. The HPLC conditions of a Kromasil C18 ODS-1（ 150 mm × 4. 6 mm,5 μm） column,methanol-isopropanol（ 9∶16,v/v） as the mobile phase at a flow rate of 0. 50 mL/min,column temperature of 25 ℃,PDA detector with detective wavelength of 210 nm were used. The polyprenols from Greek P. pinaster leaves in the range of 1. 10- 12. 10 μg had good linearity（ r = 0. 996 3）,the average recovery was 98. 21 %,and RSD was 2. 27 %（ n = 6）. The result showed that the polyprenols content of dried leaves was 0. 33 %. The method was accurate,reproducible and quick to determine the isopentenyl unit number and content of polyprenols from Greek P. pinaster leaves.